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| HPLC法测定镉、砷胁迫下水稻根系的植物螯合肽 | |
| 引用本文： | 张静,常青晓,孙传范,段桂兰,赵全志. HPLC法测定镉、砷胁迫下水稻根系的植物螯合肽[J]. 环境科学学报, 2011, 31(7): 1550-1555 |
| 作者姓名： | 张静 常青晓 孙传范 段桂兰 赵全志 |
| 作者单位： | 1. 河南农业大学水稻工程技术研究中心，河南省粮食作物生理生态与遗传改良重点实验室，郑州 450002;中国科学院生态环境研究中心土壤环境室，北京 100085 2. 河南农业大学水稻工程技术研究中心，河南省粮食作物生理生态与遗传改良重点实验室，郑州 450002 3. 中国农村技术开发中心,北京,100045 4. 中国科学院生态环境研究中心土壤环境室,北京,100085 |
| 基金项目： | 河南省高校科技创新团队支持计划项目(No.2010IRTSTHN005) |
| 摘 要： | 利用monobromobimane衍生试剂，通过反相高效液相色谱-荧光检测体系对镉、砷胁迫下水稻根系内的植物螯合肽(PCs)等巯基化合物进行形态和含量的分析测定.结果表明，采用乙腈和0.1％三氟乙酸组成的两元梯度流动相，可以将标准样品中的谷胱甘肽(GSH)和PCs等巯基化合物很好地分离.利用上述方法，能较好地分离Cd、... |
| 关 键 词： | 植物螯合肽 非蛋白巯基 反相高效液相色谱 柱前衍生 水稻 |
| 收稿时间： | 2010-09-21 |
| 修稿时间： | 2010-10-20 |
HPLC assay of phytochelatins in rice root under cadmium or arsenic stress |
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| ZHANG Jing,CHANG Qingxiao,SUN Chuanfan,DUAN Guilan and ZHAO Quanzhi. HPLC assay of phytochelatins in rice root under cadmium or arsenic stress[J]. Acta Scientiae Circumstantiae, 2011, 31(7): 1550-1555 | |
| Authors: | ZHANG Jing CHANG Qingxiao SUN Chuanfan DUAN Guilan ZHAO Quanzhi |
| Affiliation: | 1. Rice Engineering Research Center, Key Laboratory of Physiology Ecology and Genetic Improvement of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou 450002;2. Department of Soil Environmental Sciences,Research Center for Eco-env,Rice Engineering Research Center, Key Laboratory of Physiology Ecology and Genetic Improvement of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou 450002,China Rural Technology Development Center, Beijing 100045,Department of Soil Environmental Sciences,Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085 and Rice Engineering Research Center, Key Laboratory of Physiology Ecology and Genetic Improvement of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou 450002 |
| Abstract: | Phytochelatins (PCs) and other thiol-containing compounds in rice roots exposed to cadmium (Cd) and arsenic (As) were analyzed by RP-HPLC separation and fluorescence detection with monobromobimane pre-column derivatization. The results showed that GSH and PCs in the mixed standard solutions and rice roots can be separated by using ACN-0.1% TFA binary gradient elution. PC2, PC3 and PC4 were found in the rice roots that were exposed to Cd, and the concentrations of PC3 were the highest. In response to As stress, PCs in rice roots were complicated. In addition to PC2 and PC3, there are other forms of PCn that need further identification. |
| Keywords: | phytochelatins non-protein thiol RP-HPLC pre-column derivatization rice |
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