| 乙酰丙酸和前体物对Rhodobacter sphaeroides 5-氨基乙酰丙酸合成的影响 |
| |
| 引用本文： | 王俊卿,张肇铭.乙酰丙酸和前体物对Rhodobacter sphaeroides 5-氨基乙酰丙酸合成的影响[J].应用与环境生物学报,2005,11(5):611-613. |
| |
| 作者姓名： | 王俊卿 张肇铭 |
| |
| 作者单位： | 山西大学生命科学与技术学院,太原,030006 |
| |
| 基金项目： | 国家科技部攻关项目(2001BA540C)资助~~ |
| |
| 摘 要： | 球形红细菌Rhodobacter sphaeroides光合成培养过程中(接种量2g/L),分别以不同方式加入不同浓度的乙酰丙酸(LA)、前体物(甘氨酸、琥珀酸),发现LA(30mmol/L)分3次加入,甘氨酸(60mmol/L)分2次加入,可使5-氨基乙酰丙酸合成酶(ALAS)的活性(149.1U/mg)提高,而其脱水酶(ALAD)活性(143.4U/mg)降低,使ALA产量提高到33.8mmol/L.图2参9
|
| 关 键 词： | Rhodobacter sphaeroides 5-氨基乙酰丙酸 乙酰丙酸 甘氨酸 |
| 收稿时间： | 2004-09-14 |
| 修稿时间： | 2005-01-26 |
EFFECT OF LEVULINIC ACID AND PRECURSORS ON SYNTHESIS OF 5-AMINOLEVULINIC ACID IN RHODOBACTER SPHAEROIDES |
| |
| WANG Junqing,ZHANG Zhaoming.EFFECT OF LEVULINIC ACID AND PRECURSORS ON SYNTHESIS OF 5-AMINOLEVULINIC ACID IN RHODOBACTER SPHAEROIDES[J].Chinese Journal of Applied and Environmental Biology,2005,11(5):611-613. |
| |
| Authors: | WANG Junqing ZHANG Zhaoming |
| |
| Abstract: | In the photosynthetic culture of Rhodobacter sphaeroides with a suitable inoculation (2g/L), levulinic acid (LA) and precursors, glycine and succinate, were added with different concentrations. When levulinic acid (30 mmol/L) was added three times, plus the twice additions of glycine (60 mmol/L), significant enhancement of AlA production was observed. This phenomenon seemed to depend on low activity of ALAD(143.4 U/mg), which catalyzed the synthesis of tetrapyrroles from ALA and high ALA synthesis activity (149.1 U/mg), with release of suppression by twice additions of glycine. Finally ALA production increased up to 33.8 mmol/L. Fig 2, Ref 9 |
| |
| Keywords: | Rhodobacter sphaeroides 5-aminolevulinic acid levulinic acid glycine |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
