发根农杆菌T—DNA基因对3种葛属植物毛状根形态和葛根素含量的影响

野葛、山葛和三裂叶葛的离体叶片与发展根农杆菌R1601直接感染诱导出毛状根并能够合成葛根素，毛状根离体培养后均出现两种形态：一种是有较高的葛根素含量的典型毛状根，另一种是葛根素含量低但生长速度较快的愈伤组织化毛状根，两类毛状根均有rol/B基因存在，表明rolB基因对所有毛状根的诱导和生长是必需的，aux1存在于所有愈伤组织化毛状根，而在典型毛状根中的检出率为20％－50％，表明aux1基因有诱导毛状根产生愈作组织的作用，含有ags基因的毛状根葛根素含量低于不含ags基因的毛状葛根素含量，表明ags基因不利于次生代谢合成，表3参12

EFFECTS OF AGROBACTERIUM RHIZOGENE T-DNA GENES ON MORPHOLOGY AND PUERARIN CONTENT IN THREE PUERARAE PLANTS

Direct in vitro infection of leaves in vitro of three puerarae plants, Pueraria lobata (Willd.) Ohwi.,P. lobata var. montana (Lour.) van der Maesen. and P.phaseoloides (Roxb.) Benth.,with R1601 strain of Agrobacterium rhizogenes induced hairy roots with the capacity to produce puerarin. Hair roots cultured in vitro showed two morphologies: One was typical hairy roots with high puerarin content, and the other was callus-like hairy roots with low puerarin content and fast growth. Rol B gene in all typical and callus-like hairy roots indicted that rol genes were necessary for induction of all transformed roots showing typical hairy root and callus-like morphologies. Aux1 gene, existed in all callus-like hairy roots but only in 20%～50% typical hairy roots, suggested a significant role of aux genes in induction of callus on transformed roots. Puerarin content in hairy roots harboring ags gene was lower than that in those without ags gene, suggesting that the detriment of ags gene to secondary metabolite production. Tab 3, Ref 12