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耐盐石油降解菌性能及降解条件优化
引用本文:陈梅梅,钱文,田生,刘光全,郭耀峰.耐盐石油降解菌性能及降解条件优化[J].油气田环境保护,2012,22(3):7-9.
作者姓名:陈梅梅  钱文  田生  刘光全  郭耀峰
作者单位:1. 中国石油安全环保技术研究院
2. 中国石油天然气股份有限公司华北油田分公司
3. 北京林业大学
基金项目:中国石油天然气集团公司科学研究与技术开发项目(2011D-4605-0101)
摘    要:从冀东油田钻井废液中筛选分离出耐盐石油降解菌Virgibacillus sp.(简称SJ菌),其在高含盐条件下对石油具有较好的降解效果,高达56.12%左右。考察了pH值、盐度、不同N和P形态等因素对SJ菌降解石油效果的影响。结果表明:SJ菌有较宽的pH值适应范围(pH值为6~10)和较好的耐盐能力(0.5%~20%),在pH值为9及NaCl质量浓度为5%时对石油类降解效果最好,其最佳利用N源和P源分别为(NH2)2CO和KH2PO4,该研究为油田高含盐含油废液处理提供了一条新途径。

关 键 词:耐盐石油降解菌  降解性能  降解条件  优化

Optimization of Degradation Properties and Conditions of Oil-degrading Bacteria with Salt Tolerance
Chen Meimei,Qian Wen,Tian Sheng,Liu Guangquan,Guo Yaofeng.Optimization of Degradation Properties and Conditions of Oil-degrading Bacteria with Salt Tolerance[J].Environmental Protection of Oil & Gas Fields,2012,22(3):7-9.
Authors:Chen Meimei  Qian Wen  Tian Sheng  Liu Guangquan  Guo Yaofeng
Institution:1.CNPC Research Institute of Safety & Environment Technology;2.PetroChina Huabei Oilfield Company;3.Beijing Forestry University)
Abstract:Oil-degrading bacteria Virgibacillus sp(referred to as SJ bacteria) with high salt tolerance was isolated from drilling wastewater of Jidong Oilfield.It had a good degradation effect,up to about 56.12% on oil in high salt concentration conditions.This paper studied the impact of pH,salt concentration,and different nitrogen and phosphorus forms on oil degradation effects of SJ bacteria.The results showed that SJ bacteria had a wide pH range(pH 6 to 10) and a good salt tolerance(0.5% to 20%).Oil degradation rate was the highest at pH 9 and NaCl concentration of 5%.(NH2)2CO and KH2PO4 were the best N and P sources for SJ strain.Therefore this research supplied a new way for the treatment of oil wastewater with high salt concentration in the oilfield.
Keywords:oil-degrading bacteria with salt tolerance  degradation properties  degradation conditions  optimization
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