A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in Chinese agricultural samples |
| |
Authors: | Liu Yi H Chen Jian Guo Yi R Wang Chun M Liang Xiao Zhu Guo N |
| |
Institution: | Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, China. |
| |
Abstract: | A monoclonal antibody-based competitive antibody-coated enzyme-linked immunosorbent assay (ELISA) was developed and optimized for determining chlorpyrifos residue in agricultural products. The IC(50) and IC(10) of this ELISA were 3.3 ng/mL and 0.1 ng/mL respectively. The average recoveries in six agricultural products were between 79.5% and 118.0%, with the intra-assay coefficient of variation being less than 8 %. The limit of detection for all tested products was 30 ng/g. To the best of our knowledge, this assay has the best specificity among all the published research on ELISAs for chlorpyrifos. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|