邻苯二甲酸丁基苄酯的生殖毒性及其作用机制

为评价邻苯二甲酸丁基苄酯在体内的慢性毒性,以1000mg/kg、500mg/kg、250mg/kg剂量连续染毒大鼠6周、20周.结果表明:邻苯二甲酸丁基苄酯能引起雄鼠睾丸(p＜0.01)、附睾和前列腺重量的减少(p＜0.05)和肝脏肿大(p＜0.01),改变睾丸(p＜0.01)、前列腺(p＜0.05)、肝脏(p＜0.01)的组织病理学,尤其引起睾丸曲细精管萎缩、变性,各级生精细胞减少,生精上皮内生精细胞和Sertoli细胞结构明显紊乱,细胞间连接结构消失.降低血清中γ-G(p＜0.01)、ALP(p＜0.05)活性、睾酮水平(p＜0.01),增加LDH活性(p＜0.01)和FSH水平(p＜0.01),呈一定的量-效和时-效关系.结合组织病理和性激素及酶活性的改变,揭示该化合物睾丸毒性的靶细胞是支持细胞,其次是生精细胞,作用的靶点是细胞内的线粒体.邻苯二甲酸丁基苄酯对雌鼠的损害只引起子宫的增大和轻微炎症表现及血清ALP酶活性的降低(p＜0.05).充分说明了邻苯二甲酸丁基苄酯对动物的干扰有明显的性别差异,对雄性的危害要远远大于对雌性的损害.

Reproductive Toxicity and Functional Mechanism of the Environmental Hormone Butyl Benzyl Phthalate

Sustained toxicity of butylbenzyl phthalate in vivo of rat was evaluated at a dose of 1000, 500 and 250 mg/kg for 6 weeks and 20 weeks. The results show that butylbenzyl phthalate induce a decrease incidence of rats' testicle (p less 0.01), epididymides and prostate (p less 0.05). And tumefaction of liver (p less 0.01) is found. Histopathologic change is observed on testicle, prostate (p less 0.05) and liver (p less 0.01). Especially butylbenzyl phthalate can induce atrophy and denaturalization of convoluted seminiferous tubule and decreasing of spermatogenic cell on all levels. Configuration of spermatogenic cell and Sertoli cell is markedly in disorder. Conjunctive configuration between cells disappeared. In serum the activity of gamma-G (p less 0.01) and ALP (p less 0.05) and the level of testosterone (p less 0.01) are reduced. In opposition the activity of LDH (p less 0.01) and the level of FSH (p less 0.01) are increased. The alterations present hypotaxis of dose-effect and time-effect. Combining with the alteration of histopathology and sex hormone, it shows butylbenzyl phthalate's target cell is Sertoli cell and the chondriosome is its target. Butylbenzyl phthalate induces female rats accretion of uterus, slight inflammation of histopathology and decreasing of ALP's activity (p less 0.05). All the evidences adequately prove that sexual difference exists for animals' disturbance. Butylbenzyl phthalate is more harmful for male than female.