超声波-气相色谱法测定鱼肉样品中硝基苯类化合物

以鱼肉样品为研究对象,采用超声波法提取鱼肉中的硝基苯类化合物,并通过凝胶色谱进行净化、浓缩,最后用气相色谱定量分析,得到生物样品中硝基苯类化合物的检出限,同时与传统的索氏提取法进行加标回收试验比较。结果表明:用超声波提取鱼肉样品中的硝基苯类化合物方法可行,回收率稳定,并且得到检出限为0.13~2.72 ng/g,加标回收率在60%~78%,RSD在0.04%~3.2%。

Ultrasonic Gas Chromatographic Method for the Determination of Nitrobenzene Compounds in Fish Samples

Fish as samples for the study, used ultrasonic method to extract the Nitrobenzene compounds in fish, purified and concentrated by gel chromatography, and then finally analyzed by gas chromatography. The detection limits of Nitrobenzene com- pounds in biological samples were got and compared with the traditional Soxhlet extraction method in recovery experiment. Results indicate that the method of ultrasonic extraction to get the Nitrobenzene compounds in fish samples was feasible and the recovery rate was stable. The detection limits were between 0.13 ng/g - 2.72 ng/g. the standard recovery was between 60% ~ 78% and RSD was between 0.04% - 3.2%.