有机物对厌氧氨氧化生物膜反应器脱氮效能及微生物群落的影响

为考察有机物对厌氧氨氧化生物膜反应器脱氮效能的影响，采用MPN（most probable number）法和高通量测序技术，结合处理效果数据，对比分析了有无有机物影响下生物膜中微生物群落差异.试验表明：在进水有机物（COD）为30和60 mg·L^-1作用下，总氮去除率与进水COD为0 mg·L^-1时的84.10%相比较分别提高了5.08%和10.41%；COD为90 mg·L^-1时，总氮去除率降至89.05%.由MPN法和高通量测序结果可知，相对于无有机物，60 mg·L^-1有机物使反应器中反硝化菌数量增加，浮霉菌门和变形菌门丰度明显提高，且微生物群落更加丰富.有机物能影响反应器中厌氧氨氧化、反硝化脱氮效能及微生物菌落丰度，适宜的有机物浓度可使厌氧氨氧化与反硝化作用有效耦合，提高反应器的脱氮效能.本研究可为厌氧氨氧化生物膜反应器处理含有机物的实际污水提供参考价值.

Effect of organic matter on nitrogen removal efficiency and microbial community of an anammox biofilm reactor

In order to investigate the influence of organic matter on the nitrogen removal efficiency of an anammox biofilm reactor, MPN (most probable number) method and high-throughput sequencing technique were employed to compare and analyze the microbial community differences in the reactor with or without organic matter. The results showed that, the total nitrogen removal rate increased by 5.08% and 10.41%, respectively, with 30 and 60 mg·L^-1 COD in the influent, compared with the 84.10% of total nitrogen removal rate in the influent without organic matter. However, the total nitrogen removal rate decreased 89.05% under 90 mg·L^-1 organic matter. Based on MPN and high throughput sequencing results, compared with the condition of organic matter absence, 60 mg·L^-1 organic matter significantly increased the population abundance of denitrifying bacteria Planctomyces and Proteobacteria in the reactor. Organic matter could affect the efficiency of anammox and denitrification, and the abundance of microbial colonies in the reactor. The appropriate organic matter concentration could couple denitrification and anammox effectively to improve the nitrogen removal efficiency of the reactor. This study could provide a reference for anammox biofilm reactor to treat wastewater containing organic matter.