硒对体外培养大鼠胚胎的发育毒性实验研究

本实验用全胚胎培养方法探讨了硒对体外培养大鼠胚胎的发育毒性，结果表明硒对胚胎的损害有明显的剂量—反应关系，硒浓度为2.0μg/ml，首先受累及的器官是视觉系统，提示该系统是硒的主要胚胎毒性作用部位，高浓度时，硒对反映胚胎生长发育和器官形态学分化的各指标均有抑制作用，6.0μg/ml剂量组胚胎畸形率为66.7%，主要表现是无眼泡，耳泡，心包积液，腋芽小，神经管未闭和体节异常，12.0μg/ml，死胎率高达88.9%。

Study on Developmental Toxicity of Sodium Selenite in Rat Embryos in Virto

Based on the 48h culture3 of day 9.5 whole rat emtryos in serun, it has been proved that selenium would have embryotoxi-city in a dose related manner. maner.The forst selective uptake site was optic system in emtryos cultured in vitro,this result was of importance.All indexes correlated with embryonic growth and morphological differentations were inhibited at inhibited at high concentration. The incidence ofabnormal embryos was 66.7% at 6.0ug/ml. The abnormal embryos were characterized by failures of development of sen sensory placodes and limb buds, hydropericardium. open neural tube and somite abnormalities. The embryonic mortality was up to 88.9% in embryos with one to three somites exposed to 12.0ug/ml seleniun.