泉州湾河口湿地土壤硝化细菌的数量变化

为了解硝化细菌在泉州湾湿地中不同区域和不同植被土壤中的数量变化规律,应用实时荧光定量PCR方法分别对不同红树林植被土壤中的硝化杆菌属、硝化螺菌属、亚硝化单胞菌属进行鉴定和相对定量。结果表明:不同区域和不同植被土壤中三属硝化细菌相对数量有显著差异,有植被土壤高于空地和裸地;在有植被的土壤中,白骨壤下土壤最低;原生桐花树的硝化螺菌属数量明显高于其他植被土壤,互花米草的硝化杆菌属和亚硝化单胞菌属数量明显都高于红树林植被土壤。硝化细菌数量变化与植被类型和土壤的理化因子有关。

Quantitative Change of Nitrifying Bacteria at Quanzhou Bay Estuary Wetland

Quantitative change of nitrifying bacteria in different plots and vegetation types at the Quanzhou Bay estuary wetland was studied.A real-time quantitative polymerase chain reaction(RTQ-PCR) method was established to identify and quantify Nitrobacter.spp,Nitrospira.spp and Nitrosomonas.spp in the soil under different vegetation types.The results showed that there was a significant difference in quantity of the three nitrifying bacteria genera in the soil at different plots and under different vegetation types.The quantity of the three nitrifying bacteria genera in the soil under vegetations was higher than that in the new alluvial soil without plants and bared land.The quantity of the nitrifying bacteria in the soil under Avicennia marina was lowest in the soil under other vegetations.The amount of Nitrosomonas.spp in the soil under native Aegiceras corniculatum was higher than that under other mangroves.The quantity of Nitrobacter.spp and nitrosomonas.spp in the soil under Spartina alterniflora was higher than that under mangroves.Quantitative change of nitrifying bacteria is related with the vegetation types and soil phys-chemical factors.