一株具有好氧反硝化能力真菌的筛选及脱氮性能研究

利用综合筛选法从河流底泥中筛选出一株具有好氧反硝化能力的真菌,通过18S rDNA测序及形态观察,该菌被鉴定为链格孢属真菌。结果表明:该菌碳源底物利用范围广泛,能够利用多种有机碳源进行生长和反硝化。该菌对氨氮的去除主要通过该菌对氨氮的同化作用,而对亚硝酸盐和硝酸盐的去除是同化和反硝化共同作用的结果。当氨氮、亚硝酸盐和硝酸盐同时存在时,该菌优先利用氨氮,但对亚硝酸盐和硝酸盐并无明显的选择性。初始氮浓度和碳氮比对培养基pH和氮素的去除率均有一定的影响。当初始氮浓度和碳氮比合适时,该菌对3种氮素均有很高的去除效率,是一种具有应用前景的除氮菌属。

Research on Screening and Nitrogen Removal Ability of a New Isolated Aerobic Denitrifying Fungus

An aerobic denitrification fungus was isolated from river sediment through a comprehensive screening method. By the aid of 18S rDNA sequencing analysis and morphological observation, the fungus was identified as Alternaria. As a result of experiments, the fungus could use many kinds of organic carbon sources for growth and denitrification. Ammonia was removed by assimilation, while nitrite and nitrate were removed by both assimilation and denitrification. Ammonia was prior to be used when ammonia, nitrite and nitrate coexisted, but nitrite and nitrate could be used simultaneously. Initial nitrogen concentration and carbon nitrogen ratio （C/N） both had effects on nitrogen removal rate and pH change. High nitrogen removal rates were achieved when the initial nitrogen concentration and C/N were proper. Therefore, its prospect of application is cheerful.