养猪废水培养微生物絮凝剂产生菌群B-737及发酵特性

用养猪废水作为产絮菌群B-737的廉价替代培养基,研究了养猪废水COD浓度、外加草酸铵、磷酸盐的量对B-737菌群生长和产絮效果的影响,并在最优培养基下进行发酵动力学模型拟合.结果表明,该养猪废水本身具有较合适的碳氮比(COD约为3 000 mg.L-1,TN约为170 mg.L-1),无需外加碳、氮源,只添加1.6 g.L-1K2HPO4,0.8 g.L-1KH2PO4时,菌群B-737在其中发酵18～24 h便能达到1.5 g.L-1产絮量,同时对废水本身COD、TN削减率分别为61.9%和53.6%.不仅将微生物絮凝剂的培养基成本降低90%左右,也为养猪废水提供了一条新的资源化途径.用Logistic和Luedeking-Piert方程分别研究产絮菌群B-737分批发酵的生长和产絮动力学,获得了相应的动力学模式.

Piggery Wastewater Cultivating Bioflocculant-Producing Flora B-737 and the Fermentation Characteristics

Piggery wastewater was used as a cheap alternative medium for a bioflocculant-producing bacterial flora B-737. Effects of COD concent, addition of ammonium oxalate or phosphate on the cell growth and bioflocculant yield were investigated, and the fermentation kinetics was studied in the optimal culture media. The results showed that the piggery wastewater (COD was about 3000 mg·L^-1, TN was about 170 mg·L^-1) had a suitable C/N ratio for the growth and fermentation of flora B-737, with addition of only 1.6 g·L^-1 K₂HPO₄ and 0.8 g·L^-1 KH₂PO₄, flora B-737 grew well and the bioflocculant yield reached 1.5 g·L^-1, in the meantime, the COD and TN of the wastewater was reduced by 61.9% and 53.6%, respectively. Not only was the medium cost reduced by over 90%, but it was a new way to recycle piggery wastewater. In addition, the dynamic models on cell growth and flocculants formation in the fermentation process of B-737 were established according to the equation of Logistic and Luedeking-Piret, respectively.