| 32P后标记新法研究DNA-苯醌加合物的生成 |
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| 引用本文： | 蒋湘宁,刘淑芬,徐晓白.32P后标记新法研究DNA-苯醌加合物的生成[J].环境科学学报,1992,12(4):407-414. |
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| 作者姓名： | 蒋湘宁 刘淑芬 徐晓白 |
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| 作者单位： | 中国科学院生态环境研究中心 北京100085
(蒋湘宁，刘淑芬)，中国科学院生态环境研究中心 北京100085(徐晓白) |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 用以加P_1核酸酶提高灵敏度的~(32)P后标记新法研究了离体活细胞培养及试管反应形成的DNA-苯醌(BQ)加合物.4个被检加合物中有两个相应于苯醌与DNA上的胞嘧啶和鸟嘌呤作用形成的共价键加合物,其中较大的为首次发现的胞嘧啶-苯醌加合物.测得其相对标记率(RAL)试管条件为6.70×10~(-8)和8.93×10~(-9),细胞培养为8.23×10~(-9)和6.91×10~(-9),达到了在基因水平上研究DNA加合物的基本要求.
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| 关 键 词： | 核酸酶 磷32 DNA－BQ |
| 收稿时间： | 1991/2/10 0:00:00 |
BENZOQUINONE DNA ADDUCTS ANALYZED BY NUCLEASE P1 MEDIATED 32P-POSTLABELLING |
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| Jiang Xiangning,Liu Shufen and Xu Xiaobai.BENZOQUINONE DNA ADDUCTS ANALYZED BY NUCLEASE P1 MEDIATED 32P-POSTLABELLING[J].Acta Scientiae Circumstantiae,1992,12(4):407-414. |
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| Authors: | Jiang Xiangning Liu Shufen and Xu Xiaobai |
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| Institution: | Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing 100085,Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing 100085 and Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing 100085 |
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| Abstract: | With the super sensitivity, high reproducibility and accuracy, nuclease P1 mediated 32P-postlabelling version has been used successfully to analyze the DNA-BQ adducts formed from in vitro cultured cells, reaction of benzoquinone with calf thymus DNA and nucleoside monophposphates. It has been shown that the major one, which consists of more than 70% of the total radioactivities of DNA-BQ adducts detected, is from the deoxycytidine (dC) modified by benzoquinone while a minor one from deoxyguanosine (dG). The method is ca pable of detecting 1 adduct in 109-1010 DNA bases. |
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| Keywords: | P_1 mediated ~(32)P-postlabelling DNA-BQ aducts dC-BQ ad duct dG-BQ adduct |
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