| 1,2,4-三氯苯矿化菌的鉴定与功能分析 |
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| 引用本文： | 王芳,U.Drfler,M.Schmi,S.Grundmann,J.C.Munch,蒋新,R.Schroll.1,2,4-三氯苯矿化菌的鉴定与功能分析[J].环境科学,2007,28(5):1082-1087. |
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| 作者姓名： | 王芳 U.Drfler M.Schmi S.Grundmann J.C.Munch 蒋新 R.Schroll |
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| 作者单位： | 1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京,210008
2. Institute of Soil Ecology,GSF-NationalResearch Center for Environment and Health,85764 Neuherberg,Germany |
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| 基金项目： | 国家杰出青年科学基金项目（40325001）;国家重点基础研究发展规划（973）项目（2002CB410805）;中国科学院海外合作伙伴计划项目（CXTD-Z2005-4） |
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| 摘 要： | 采用同位素示踪法从氯苯污染土壤中筛选到能高效降解1,2,4-三氯苯(1,2,4-TCB)为CO2的细菌E3和F2,根据其表型特征、16S rDNA序列和荧光原位杂交(FISH)分析,鉴定E3和F2为博德特氏菌（Bordetella sp.）.E3和F2的16S rDNA序列相似性为100%;它们与菌株Bordetella sp. QJ2-5同源性最高,与Bordetella petrii(GDH030510)最接近,同源性分别为100%和99.4%.E3和F2能在含1,2,4-TCB的无机盐培养基中彻底降解1,2,4-TCB生成CO2,30 d降解率达到90%以上,其中58%和46%矿化为CO2,同时形成一定量的微生物生物量.
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| 关 键 词： | 同位素示踪法 三氯苯 生物降解 博德特氏菌属(Bordetella sp.) 16S rDNA 荧光原位杂交(FISH) |
| 文章编号： | 0250-3301（2007）05-1082-06 |
| 收稿时间： | 2006/7/20 0:00:00 |
| 修稿时间： | 2006-07-202006-11-08 |
Identification of 1,2,4-Trichlorobenzene-Mineralizing Bacteria and Their Function Analysis |
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| Wang Fang,D?rfler U,Schmid M,Grundmann S,Munch J C,Jiang Xin,Schroll R.Identification of 1,2,4-Trichlorobenzene-Mineralizing Bacteria and Their Function Analysis[J].Chinese Journal of Environmental Science,2007,28(5):1082-1087. |
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| Authors: | Wang Fang Dörfler U Schmid M Grundmann S Munch J C Jiang Xin Schroll R |
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| Institution: | 1 .State Key laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; 2. Institute of Soil Ecology, GSF-National Research Center for Environment and Health, 85764 Neuherberg, Germany |
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| Abstract: | Two strains, E3 and F2, capable to mineralize 1,2,4-trichlorobenzene(1,2,4-TCB) were isolated from a chlorinated benzenes contaminated soil using 14C-1,2,4-TCB as carbon source. They were identified by their 16S rDNA coding genes and fluoresence in situ hybridization (FISH) analysis as members of the genus Bordetella. A similarity of 100% were observed between strains E3 and F2 with their 16S rDNA sequences. They had the highest homology of 100% with Bordetella sp. QJ2-5 and the closest relation to described species, Bordetella petrii (GDH030510) with a similary of 99.4%. Strains E3 and F2 could degrade about 90% of 1,2,4-TCB and mineralize 58% and 46% of 1,2,4-TCB to CO2 within 30 days in mineral liquid cultures, respectively. Biomass was formed during the mineralization process. |
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| Keywords: | 14C-labeled TCB biodegradation Bordetella sp 16S rDNA fluorescence in situ hybridization(FISH) |
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