除虫菊酯和迷迭香对大鼠大脑突触体ATP酶的活性影响

除虫菊酯越来越广泛地被应用于农业和家庭昆虫防治,主要通过作用于膜结合蛋白而对动物起神经毒性.迷迭香因其抗氧化功能而被应用于很多商业化的除虫菊酯产品中.本实验以大鼠大脑突触体ATP酶为研究对象,对除虫菊酯和迷迭香的药理学进行了研究.用Percoll梯度离心法分离突触体,通过检测无机磷的量来测定总ATP酶和Mg2 -ATP酶活性.结果表明,除虫菊酯在浓度为10 μmol/L时总ATP酶和Mg2 -ATP酶分别降低到对照的80.3%和46.9%.迷迭香在浓度为0.3～30 μmol/L时几乎不影响ATP酶活性,当浓度上升到3 000 μmol/L时,总ATP酶活性降低到66.8%,而Mg2 -ATP酶活性降低到54.5%. 10 μmol/L 除虫菊酯和30 μmol/L迷迭香混合物引起总ATP酶和Mg2 -ATP酶分别降低到72.9%和33.4%.结论: 1) 除虫菊酯能抑制大鼠大脑ATP酶活性; 2) 迷迭香只在高浓度下才对ATP酶有抑制作用; 3) 迷迭香能增强除虫菊酯对ATP酶的抑制作用.图3参19

Effect of Pyrethrins and Rosemary on the ATPase Activities of Rat Cerebral Synaptosome

Pyrethrins are the widely used insecticides in agriculture and households, and they act on integral membrane proteins, causing neurotoxic effects on animals. Rosemary, as an antioxidant, is developed to improve the stability of pyrethrins and applied for many commercially pyrethrinrelated products. This paper aimed to study the effects of pyrethrins and rosemary, as well as their combination on the cerebral synaptosome ATPase of rat. The Percoll gradient method was used to isolate synaptosomes. Total ATPase and Mg2+-ATPase activities were analyzed by determining the amount of inorganic phosphate. The results indicated that the activities of total ATPase and Mg2+-ATPase in 10 μmol/L pyrethrins were reduced to 80.3% and 46.9%, respectively, compared with CK. However, rosemary at concentration of 0.3 to 30 μmol/L showed little effect on ATPase, while at the concentration of 3 000 μmol/L, the total ATPase and the Mg2+-ATPase activities were decreased to 66.8% and 54.5%, respectively. The mixture of 10 μmol/L pyrethrin and 30 μmol/L rosemary decreased the activities of the total ATPase and Mg2+-ATPase to 72.9% and 33.4%, respectively. It is concluded that pyrethrins can inhibit ATPase activity of rat cerebral synaptosome dramatically; rosemary inhibits ATPase only at high concentration; and rosemary slightly enhances inhibition of pyrethrins on ATPase activity. Fig 3, Ref 19