孕哺期全氟辛烷磺酸染毒对大鼠海马细胞钙稳态的影响

为了阐明孕哺期全氟辛烷磺酸(perfluorooctane sulfonate,PFOS)染毒对大鼠及其仔鼠海马细胞钙稳态的影响,将妊娠Wistar大鼠30只,从妊娠第1天开始对实验组分别以72 mg·kg-1(low,L)、14.4 mg·kg-1(high,H)(以饲料中的PFOS计)的PFOS进行染毒至仔鼠生后3...

Effect of Perinatal PFOS Exposure on Intracellular Free Calcium Concentration in Hippocampus of Rats

Effect of perinatal exposure to PFOS on calcium concentration in hippocampus of Wistar rats was investigated.The pregnant rats were exposed to PFOS with doses of 0 mg·kg-1(control,C),7.2 mg·kg-1(low,L) or 14.4 mg·kg-1(high,H) food from pregnancy day 1 to postnatal day 35.The concentration of PFOS was detected by HPLC/MS-MIS,[Ca2+]i was determined with Fura-2/AM fluorescence probe.The results show that PFOS can be concentrated in hippocampus of rats.[Ca2+]i in 7.2 and 14.4 mg·kg-1 PFOS-treated group were(175.4±11.9),(313.4±18.7) nmol·L-1,which were significantly higher than(155.6±11.9) nmol·L-1 in the control(p<0.05).At PND1,[Ca2+]i in the high group was significantly higher than control(p<0.01).[Ca2+]i in the HH group was significantly higher than that in CC and LL at PND28 and PND35(p<0.01),respectively.[Ca2+]i in the HC group was obviously higher than that in the LC and CC group at PND28 and 35(p<0.01),respectively.In addition,with the days increased,[Ca2+]i in the PFOS exposure group increased significantly(p<0.05).The results show that [Ca2+]i increased in hippocampus of rats not only in the adults,but also in pups exposure to PFOS with different times,which might have a neurotoxic effect on central nervous system.