| 青蒿无性系中青蒿素生物合成的相关因素 |
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| 引用本文： | 张龙,叶和春,李国凤.青蒿无性系中青蒿素生物合成的相关因素[J].应用与环境生物学报,2004,10(3):277-280. |
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| 作者姓名： | 张龙 叶和春 李国凤 |
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| 作者单位： | 中国科学院植物研究所光合作用与环境分子生理学重点实验室,北京,100093 |
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| 基金项目： | 科技部技术创新基金资助项目(99C26211100342)~~ |
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| 摘 要： | 通过诱导丛生芽的方法得到青蒿高产无性克隆系S1,并用S1进行了青蒿素生物合成相关因素的研究 .发现在Hoagland培养液中添加 0 .4 4mg/L浓度的ZnSO4或者 5 .72mg/L的HBO3 时 ,能有效提高青蒿素的含量 .研究表明 ,青蒿素含量和青蒿各株系间的遗传差异存在很大关系 ,RAPD分析的结果表明 ,用OPA15能够在青蒿高产株系中扩增到OPA15 10 0 0 这一特异性片段 .另外 ,对青蒿素含量和青蒿生殖生长关系的研究表明 ,青蒿素含量最高的发育时期是现蕾期 .图 7表 1参 19
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| 关 键 词： | 青蒿 无性克隆系 青蒿素 遗传差异 RAPD 生殖生长 |
| 修稿时间： | 2003年4月23日 |
RELATED FACTORS OF ARTEMISININ BIOSYNTHESIS IN CLONE STRAIN OF ARTEMISIA ANNUA L. |
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| Abstract: | By inducing shoot cluster, the high yield clone strain of Artemisia annua L. named S 1 was obtained. The effects of different concentrations of ZnSO 4 , HBO 3 on artemisinin content in S 1 cultured in Hoagland medium were tested. Artemisinin content in S 1 effectively increased when ZnSO 4 (0.44 mg/L) or HBO 3 (5.72 mg/L) was added to the Hoagland medium respectively. There were close relation between artemisinin content and the development stages, glandular trichomes and genetic variation of Artemisia annua L. A special sequence amplified by RAPD arbitrary primer OPA15 from high artemisinin strains was obtained. At the bud stage artemisinin content was highest in the whole life of Artemisia annua L. Fig 7, Tab 1, Ref 19 |
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| Keywords: | Artemisia annua L clone strain artemisinin genetic variation RAPD reproductive growth |
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