活性炭纤维固定化菌对微囊藻毒素MC-LR的去除研究

研究了藻蓝蛋白提取过程中微囊藻毒素MC-LR的释放分布规律,并用活性炭纤维对一株微囊藻毒素降解菌株进行了固定化,考察了不同活性炭纤维预处理方法、活性炭纤维用量、pH值、温度以及MC-LR浓度对固定化藻毒素降解菌去除MC-LR的影响.结果表明,藻蓝蛋白提取过程中MC-LR主要分布在超滤滤液中,占MC-LR总含量的81.2%.固定化藻毒素降解菌去除 MC-LR的效率明显高于非固定化藻毒素降解菌. 藻毒素降解菌用(1+9)盐酸预处理后的活性炭纤维固定化,其去除效果最佳.MC-LR去除的最适条件为:活性炭纤维用量为10g/L,温度为35℃, pH值为8.0.固定化藻毒素降解菌对pH值,温度具有一定的耐受性,能够在pH5~pH9、10℃~35℃范围内有效地去除MC-LR.

Removal of microcystin-LR by a microcystin-degrading strain immobilized by activated carbon fiber

Distribution of MC-LR during the extraction of phycocyanin was studied. A microcystin-degrading strain was immobilized by activated carbon fiber (ACF), and the effect factors, such as the pretreatment methods of activated carbon fiber, dosage of activated carbon fiber, pH value, temperature and the initial concentration of MC-LR were investigated. The results showed that 81.2% of MC-LR existed in the ultrafiltrate during the phycocyanin extraction. The immobilized strain presented significantly better performance in MC-LR removal than the non-immobilized strain. The performance of ACF which was pretreated with (1+9) HCl was much better than others. The optimum removal conditions were as follows: dosage of activated carbon fiber was 10g/L, the temperature was 35℃, the pH value was 8.0, and the initial concentration of MC-LR was 13.6mg/L. Therefore, immobilized strain showed a certain tolerance towards the pH value and the temperature. And MC-LR could be removed effectively in the range of pH5 to pH9, 10℃~35℃.