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Improved silica gel cleanup method for organophosphorus pesticides
Affiliation:2. U.S. Environmental Protection Agency, Environmental Research Laboratory, Gulf Breeze, FL 32561 USA;3. Computer Science Corporation, Environmental Protection Agency Research Laboratory, Gulf Breeze, Fl 32561 USA;1. Academy of Scientific and Innovative Research (CSIR–CFTRI campus, Mysore), India;2. Plant Cell Biotechnology Department, CSIR–CFTRI, Mysore-570020, India;1. Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, PO Box 1068, Blindern, N-0316 Oslo, Norway;2. National Research Institute of Chinese Medicine, Taipei, Taiwan;3. Institute of Biophotonics, National Yang-Ming University, Taipei 112, Taiwan;4. Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Norway;1. Department of Applied Chemistry, Yamaguchi University, Ube, Yamaguchi 755-8611, Japan;2. Department of Chemistry, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8511, Japan;1. Pharmacy College of Xinjiang Medical University, Urumqi, 830011, China;2. College of Medical Engineering and Technology, Xinjiang Medical University, Urumqi, 830011, China;3. Central Laboratory of Xinjiang Medical University, Urumqi, 830011, China
Abstract:Quantitative recovery of some organophosphorus pesticide residues has not been possible with existing silica gel-cleanup procedures. We have developed a modification that permits quantitative recovery of all organophosphorus pesticides tested except those with a carbamate functional group. The method uses a 3.5 g silica gel column with a 1% acetic acid wash to condition the column prior to the addition of the sample. Percentage recovery and standard deviation of compounds such as phorate and disulfoton are 96 (5.6) and 98 (1.0) respectively. Recoveries range from 92 to 101% for the 11 compounds tested.
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