| 高效含氯有机化合物降解工程菌的构建研究 |
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| 引用本文： | 许燕滨 江霞. 高效含氯有机化合物降解工程菌的构建研究[J]. 重庆环境科学, 2001, 23(2): 46-48,51 |
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| 作者姓名： | 许燕滨 江霞 |
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| 作者单位： | [1]广东工业大学环境工程系，广州510090 [2]广东省食品工业 |
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| 基金项目： | 华南理工大学制浆造纸国家重点实验室资助广东省环保局攻关项目 |
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| 摘 要： | 采用原生质体融合技术，将两株具有含氯有机化合物降解特性的菌－-1T和RB－1融合构建成一株高效降解含氯有机化合物工程菌。实验得出：当溶菌酶浓度为1.5mg/ml，酶解60min时，假单胞菌和诺卡氏菌的原生质体形成率分别为33.3%和44.4%；在30％PEG6000助融下10min二者的融合率为0.3%。应用于造纸漂白废水，融合菌去除CODCr和TCL的能力 比混合菌分别提高了72.05%和190%.
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| 关 键 词： | 工程菌 原生质体融合 含氯有机化合物 废水处理 |
| 文章编号： | 1001-2141(2001)02-0046-04 |
Study on the Construction of Engineering Bacteria Degrading Organic Chloride |
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| Xu Yanbin ,Jiang Xia. Study on the Construction of Engineering Bacteria Degrading Organic Chloride[J]. Chongqing Environmental Science, 2001, 23(2): 46-48,51 |
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| Authors: | Xu Yanbin Jiang Xia |
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| Affiliation: | Xu Yanbin 1,Jiang Xia 2 |
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| Abstract: | By the technique of protoplast fusion,interspecific fusants between Pseudomanos SY l and Norcardia RB 1 had been obtained.The superior conditions of protoplast formation,protoplast fusion and wastewater treatment using the fusants were studied.It is better to prepare the protoplast by using lysozyme 1.5mg/ml,10min.The protoplast formation rates of Pseudomamos and Norcardia were 33.3% and 44.4% respectively.The fusion was carried out in NSM containing 30% PEG(MW6000) and the fusion frequency was about 0.3%.At last,the stable fusants were sued to treat the bleaching wastewater.It is obtained that the removal rates of COD Cr and TCL are respectively 72.05% and 190% higher than that of the mixed parent strains. |
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| Keywords: | Engineering bacterium Protoplast fusion Organic chloride. |
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