不同生长期的Pseudomonas putida 5-x细胞对工业废水中Cu~(2+)的吸附

研究了不同生长阶段的Cu2 + 吸附细菌Pseudomonasputida 5 x对Cu2 + 的吸附容量 .结果表明在低硫培养基中 ,当P putida 5 x细胞生长刚进入对数生长期时 ,单位细胞对Cu2 + 的吸附溶量最低 .而当生长进入稳定生长期的后期或死亡期的早期时 ,单位细胞对Cu2 + 的吸附量最大 .实验结果还表明 ,高浓度的Cu2 + 对对数期细胞的吸附能力有一定的抑制 ,而对死亡期的细胞影响较小 .早期研究已证明细胞对重金属离子的吸附和细胞表面某些对重金属离子具有亲和性的功能基团 (如某些膜蛋白及羰基化合物 )有关 .利用透视电子显微镜观察了不同生长阶段的细胞表面结构及对铜离子的吸附效果 ,发现生长后期细胞表面吸附有较多的Cu2 + ,这表明生长后期的细胞表面可能含有较多的这类功能基团

Biosorption of Pseudomonas putida 5-x cell in different growth phases on Cu~(2+) removal in industry effluent

Biosorption using microorganism as biosorbent for the removal of heavy metal ions in industrial waste effluent is a useful method The biosorption capacity to Cu 2+ of P putida 5 x harvested in different growth phase was investigated The experimental results illustrated that the Cu 2+ adsorption capacity of P putida5 x cultured in sulphate limiting medium (SLM) reached minimum in log growth phase, and reached maximum in late stationary growth phase to early death phase Higher concentration Cu 2+ inhibited adsorption capacity of early cell to Cu 2+ , but no obvious affection to late cell The results of TEM analysis implied that Cu 2+ was mainly accumulated on the cell surface, and late period cells adsorbed more Cu 2+ on cell surface than early period cells due to late cell has more active groups of Cu 2+ adsorption on cell surface than early cell