| FISH法检测生物膜中硝化细菌流程的建立及优化 |
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| 引用本文： | 张勇,宋吟玲,史俊,陈宇.FISH法检测生物膜中硝化细菌流程的建立及优化[J].江苏环境科技,2009,22(4):34-37. |
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| 作者姓名： | 张勇 宋吟玲 史俊 陈宇 |
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| 作者单位： | 苏州科技学院环境科学与工程学院,江苏,苏州,215011 |
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| 摘 要： | 通过试验筛检荧光原位杂交（FISH）技术在检测反应器生物膜中硝化细菌时适合的实验条件。结果显示，FISH技术检测生物膜中硝化细菌时．较好的预处理条件和杂交条件为：样品在热固定前不需1xPBS清洗；检测生物膜中硝化细菌的实验条件：①硝酸细菌：杂交温度46℃，杂交时间5h，洗脱液中c（NaCl）为55mmol／L；（爹亚硝酸细菌：杂交温度46℃，杂交时间3h，洗脱液中c（NaCl）为30～40mmol/L。
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| 关 键 词： | 硝化细菌 荧光原位杂交 杂交优化 |
Establishment of Experimental Conditions and Optimization of Fluorescence in situ Hybridization Assay for Detecting Nitrifying Bacteria in Biofilm |
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| ZHANG Yong,SONG Yin-ling,SHI Jun,CHEN Yu.Establishment of Experimental Conditions and Optimization of Fluorescence in situ Hybridization Assay for Detecting Nitrifying Bacteria in Biofilm[J].Jiangsu Environmental Science and Technology,2009,22(4):34-37. |
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| Authors: | ZHANG Yong SONG Yin-ling SHI Jun CHEN Yu |
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| Institution: | ZHANG Yong, SONG Yin-ling, SHI Jun, CHEN Yu |
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| Abstract: | Experimental conditions of fluorescence in situ hybridization （FISH） for detecting nitrifying bacteria in biotilm is optimized through test. Results show that the optimized pretreatment and hybridization conditions for detecting nitrifying bacteria include： Samples aren＇t cleaned by 1xPBS before heat fixed. Hybridization conditions of detecting nitrifying bacteria in biofilm are as follows： ①Nitrobacter： temperature is 46 ℃, hybridization time is 5 h, and sodium chloride concentration in the eluate is 55 mmol/L. ②Nitrosomonas： temperature is 46 ℃, hybridization time is 3h , and sodium chloride concentration in the eluate is 30 - 40 mmol/L. |
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| Keywords: | Nitrifying bacteria Fluorescence in situ hybridization （FISH） Hybrid optimization |
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