Cultures of the pelagic cyanophytes Trichodesmium erythraeum and T. thiebautii in synthetic medium |
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Authors: | K. Ohki J. G. Rueter Y. Fujita |
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Affiliation: | (1) National Institute for Basic Biology, 444 Okazaki, Aichi, Japan;(2) Biology Department, Portland State University, P.O. Box 751, 92707 Portland, Oregon, USA |
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Abstract: | Trials for determination of culture conditions for the marine cyanophytes of Trichodesmium erythraeum and T. thiebautii were made with use of a synthetic medium. The Aquil medium, either with or without combined nitrogen, brought about stable growth of the two strains, T. erythraeum and T. thiebautii. However, they failed to grow in an ASP7 medium. The failure was found to be due to the toxic effect of Tris-aminomethane, the pH-buffer in this medium. Two important chemical conditions for the stable growth of Trichodesmium spp. were revealed. (1) Stable growth was supported by Ca2+ at high concentrations; in a concentration lower than 0.9 mM, cell-lysis promptly occurred, while the cells could grow without cell-lysis at Ca2+ concentrations higher than 7.5 mM even at a salinity as low as 19 S. Ca2+ is probably essential for the osmotic regulation in this organism. (2) Phosphate-toxicity at high concentrations was at least partly due to heavy metal(s) contaminating the reagent of inorganic phosphate. After treatment with a Chelex-100 column, phosphate concentration could be increased up to four times the previous concentrations without toxicity. |
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