Effects of Zn2+ on root growth,cell division, and nucleoli of Allium cepa L. |
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作者姓名: | Liu Donghu Jiang Wusheng Wang Chunli Zhai Lin |
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作者单位: | (Department of Biology;Tianjin Normal University;Tianjin 300074;China),(Department of Biology;Tianjin Normal University;Tianjin 300074;China),(Department of Biology;Tianjin Normal University;Tianjin 300074;China),(Xinhua High School;Tianjin 300204;China) |
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基金项目: | The study was supported by a grant for junior scientists from Tianjin Normal University, Tianjin, China |
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摘 要: | The effects of different concentrations of Zn2+ion on root growth,cell division,and nucleoli of Allium cepa were studied. The test Zn2+ ion concentration was made up from zinc sulphate (ZnSO4. 7H2O) ranging from 10-7 to 10-2 mol/L. The solutions were prepared in tap water (pH =6. 5).The results indicated that Zn2+ could obviously inhibit root growth at concentrations from 10-4)to 10-2 mol/L.Roots treated with zinc sulphate showed the presence of c-mitosis, anaphase bridges,including sticky and fluidized bridges (at 10-3 to 10-2 mol/L) , chromosome stickiness, irregularly shaped nuclei, broken nuclei and micronuclei. A toxicity effect was also observed on the nucleoli using silver staining technique after 48h of treatment with 10-4to 10-2 mol/L Zn2+, e. g,the nucleolar particulate material scattered around the nucleoli in the nucleus of root tip cells.
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关 键 词: | Allium cepa mitosis chromosome aberration nucleoli silver staining technique. |
收稿时间: | 1994/5/11 0:00:00 |
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