荷花和睡莲种植水对铜绿微囊藻生长的抑制作用研究

在实验室条件下,通过测定培养液中铜绿微囊藻的藻细胞浓度和叶绿素a含量,研究了具有较高观赏价值的水生植物荷花和睡莲种植水对铜绿微囊藻生长的抑制效应.结果表明,不同浓度的荷花和睡莲种植水对铜绿微囊藻生长的抑制作用不尽相同,表现为明显的低促高抑现象;而连续滴加荷花和睡莲种植水对铜绿微囊藻的生长也有较明显的抑制作用,使藻细胞基本失去了进行正常光合作用的能力,其中睡莲种植水的抑制效果比荷花的更明显;另外,扩大实验中通过测定铜绿微囊藻的SOD活性、MDA积累量表明,藻细胞在共培养过程中受到了胁迫和伤害.灭菌方法对种植水的抑藻试验也有影响,不能采用高温灭菌来代替微膜过滤,说明荷花和睡莲分泌物中含有热不稳定性的物质.

Inhibitory Effects of Liquor Cultured with Nelumbo nucifera and Nymphaea tetragona on the Growth of Microcystis aeruginosa

The inhibitory effects of liquor cultured with Nelumbo nucifera and Nymphaea tetragona on the growth of Microcystis aeruginosa have been investigated by measuring the cell number and the chlorophyll a content of Microcystis aeruginosa of culture in the laboratory. The results showed that the inhibitory effects on the growth of Microcystis aeruginosa with different concentration of the liquor cultured with Nelumbo nucifera and Nymphaea tetragona were dissimilar, and an evident phenomenon appeared that low concentrations could promote the growth of Microcystis aeruginosa. However, the inhibitory effects on the growth of Microcystis aeruginosa with continuous liquor cultured with Nelumbo nucifera and Nymphaea tetragona were obvious, which made the algal cell almost lose the capability of photosynthesis, and the inhibitory effects of liquor cultured with Nymphaea tetragona were better than those of Nelumbo nucifera. The scale-up experiment demonstrated that the algal cell received menace and damage by measuring the activities of peroxidase (SOD) and the accumulated contents of malondialdehyde (MDA) of Microcystis aeruginosa in the co-culture. Sterilization methods influenced the algal growth inhibition experiment so that high temperature couldn't replace micropore filter, which explained that the matter excreted by Nelumbo nucifera and Nymphaea tetragona may contain thermally-instabe matter.