| 嗜盐高效降酚菌株Halomonas sp.H17的筛选及降解苯酚特性 |
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| 引用本文： | 赵娜娜,许继飞,宋晓雪,田鹏,丁舒心,赵吉.嗜盐高效降酚菌株Halomonas sp.H17的筛选及降解苯酚特性[J].环境科学学报,2019,39(2):318-324. |
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| 作者姓名： | 赵娜娜 许继飞 宋晓雪 田鹏 丁舒心 赵吉 |
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| 作者单位： | 内蒙古大学环境污染控制与废物资源化自治区重点实验室,呼和浩特010021;内蒙古大学生态与环境学院,呼和浩特010021;上海师范大学天华学院,上海,201815;内蒙古大学生态与环境学院,呼和浩特,010021 |
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| 基金项目： | 内蒙古自治区自然科学基金（No.2016MS0305） |
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| 摘 要： | 从巴丹吉林沙漠盐湖沉积物中分离获得1株在高盐环境下高效降解苯酚菌H17.分析了H17生理生化特性、16S rDNA基因序列、苯酚降解特性及动力学,结果表明,H17属于盐单胞菌属(Halomonas sp.),能在0～20%的盐度下有效降解苯酚,每升外加适量的碳源(葡萄糖浓度0.8 g)和复合氮源(KNO_3 1 g、NH_4Cl 5 g、酵母提取物0.2 g和胰蛋白胨0.2 g)能够促进H17的生长及降解苯酚能力.在温度为30℃、pH 7～8、盐度5～10%的条件下,H17均能高效降解苯酚,最高降解率可达到88.5%.该菌株降解苯酚动力学符合Halane模型,经拟合其生长参数为μ_(max)=0.31 h~(-1),K_S=191.63 mg·L~(-1),K_i=683.05 mg·L~(-1).研究显示H17具有在高盐环境下降解和耐受苯酚的能力,同时对环境有较强的适应性,体现出其在高盐含酚废水实际处理中具有良好的应用价值.
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| 关 键 词： | 嗜盐菌 Halomonas sp. 苯酚 生物降解 动力学 |
| 收稿时间： | 2018/7/19 0:00:00 |
| 修稿时间： | 2018/9/30 0:00:00 |
Screening and phenol-degrading characteristics of a highly efficient phenol-degrading halophilic bacterial strain Halomonas sp. H17 |
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| ZHAO Nan,XU Jifei,SONG Xiaoxue,TIAN Peng,DING Shuxin and ZHAO Ji.Screening and phenol-degrading characteristics of a highly efficient phenol-degrading halophilic bacterial strain Halomonas sp. H17[J].Acta Scientiae Circumstantiae,2019,39(2):318-324. |
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| Authors: | ZHAO Nan XU Jifei SONG Xiaoxue TIAN Peng DING Shuxin and ZHAO Ji |
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| Institution: | 1. Inner Mongolia Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, Hohhot 010021;2. School of Ecology and Environment, Inner Mongolia University, Hohhot 010021,1. Inner Mongolia Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, Hohhot 010021;2. School of Ecology and Environment, Inner Mongolia University, Hohhot 010021,Shanghai Normal University Tianhua College, Shanghai 201815,School of Ecology and Environment, Inner Mongolia University, Hohhot 010021,School of Ecology and Environment, Inner Mongolia University, Hohhot 010021 and 1. Inner Mongolia Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, Hohhot 010021;2. School of Ecology and Environment, Inner Mongolia University, Hohhot 010021 |
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| Abstract: | |
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| Keywords: | Halophilic bacteria Halomonas sp phenol biodegradation kinetics |
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