In Vitro Biodegradation of Polyester-Based Plastic Materials by Selected Bacterial Cultures |
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Authors: | Joris Mergaert Kurt Ruffieux Christophe Bourban Virginie Storms Wim Wagemans Erich Wintermantel Jean Swings |
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Affiliation: | (1) Laboratorium voor Microbiologie, Vakgroep Biochemie, Fysiologie en Microbiologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium;(2) Eidgenössische Technische Hochshule Zürich, Wagistrasse 6, CH-8952 Schlieren, Switzerland;(3) BCCM/LMG Culture Collection, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium |
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Abstract: | A simple and rapid in vitro test was designed for the assessment of the biodegradation of polyester-based plastics by selected biodegrading bacterial strains. Variovorax paradoxus LMG 16137 was used for the degradation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Acidovorax avenae subsp. avenae LMG 17238 fo the synthetic-based polyesters poly(-caprolactone) (PCL), poly(butylene succinate-co-butylene adipate), and a starch-PCL blend. Degradation by the bacteria was studied in liquid medium with the plastics (films, granules, and injection-molded test bars) as sole sources of carbon. Degradation was followed through gravimetry, growth of the culture, and tensile testing. The effects of incubation time, inoculum density, aeration, incubation temperature, and pH of the medium on the mass loss were investigated and conditions optimized. The test allowed to obtain reproducible results on the mass loss of plastic samples in less than 3 weeks and yielded excellent partially degraded samples for further analysis. |
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Keywords: | Variovorax paradoxus Acidovorax avenae subsp. avenae poly(3-hydroxybutyrate-co-3-hy-droxyvalerate) poly( /content/q488rx6m65340k32/xxlarge949.gif" alt=" epsi" align=" BASELINE" BORDER=" 0" >-caprolactone) poly(butylene succinate-co-butylene adipate) in vitro biodegradation |
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