基于析因设计的多种重金属对发光菌联合毒性研究

文章采用析因设计的方法,研究沉积物水浸提液掺杂的多种重金属Cu、Cd、Zn、Pb和Ni对发光菌的联合毒性。通过对试验结果进行方差分析和多元线性回归,建立了重金属浓度和发光菌发光抑制率的关系模型:T=97.983Cu+41.111Cd-27.297Zn+129.869Pb+16.715Ni-5 803.639Cu*Cd+2 270.182Cd*Zn-6 411.870Cd*Pb-675.052Cd*Ni-3 108.544Pb*Ni+15 9552.434Cd*Pb*Ni+274 718.434Zn*Pb*Ni。模型分析结果表明:Zn对联合毒性产生负的影响,Cu、Cd、Pb、Ni对联合毒性具有正的贡献;Cd*Zn二元交互作用和Cd*Pb*Ni、Zn*Pb*Ni三元交互作用表现为协同作用,Cu*Cd、Cd*Pb、Cd*Ni、Pb*Ni二元交互作用表现为拮抗作用,而重金属四元和五元交互作用对联合毒性的贡献不显著。上述重金属联合毒性的作用可为沉积物中重金属复合污染控制提供参考。

Joint Toxicity of Multi-Heavy-Metal to Photobacterium Phosphoreum Based on Factorial Design

Solution of aqueous extract of sediment samples which were taken from Jilin City's section of Songhua River,was doped with a number of heavy metals,such as Cu,Cd,Zn,Pb and Ni,for the purpose of investigating joint toxicity of multiple heavy metals to photo-bacteria Photobacterium Phosphoreum.This paper described the experiment in which method of factorial design was used,and the experimental results were analyzed using variance analysis and multiple linear regressions,which led to a model that could depict the relationship between heavy metal concentration and the photo-bacteria inhibition ratio.The modeling results showed the synergetic affects of heavy metals in different groups(binary and ternary)on the joint toxicity to photo-bacteria,which could provide references for the abatement of combined pollution of heavy metals in river sediment.