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| 一株受二噁英类化合物诱导表达绿色荧光蛋白的胃癌细胞系的建立 | |
| 引用本文： | 汪畅,王英,雷垚,张捷,郑明辉,梁勇. 一株受二噁英类化合物诱导表达绿色荧光蛋白的胃癌细胞系的建立[J]. 生态毒理学报, 2008, 3(3): 244-249 |
| 作者姓名： | 汪畅 王英 雷垚 张捷 郑明辉 梁勇 |
| 作者单位： | 1. 华中农业大学资源与环境学院,农业部亚热带农业环境重点实验室,武汉,430070 2. 江汉大学医学院,武汉,430056 3. 中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京,100085 4. 江汉大学医学院,武汉,430056;中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京,100085 |
| 基金项目： | 武汉市青年科技晨光计划项目(No. 20055003059-11)；国家自然科学基金项目(No. 40332023) |
| 摘 要： | 将人工合成的5个相连的XRE(Xeobiotic response element)和minimal CMV(Human cytomegalovirus)启动子,插入载体pEGFP-1,构建得到载体pXRE5-EGFP.以pXRE5-EGFP转染人胃癌细胞SGC-7901,经过筛选得到一株受2,3,7,8-四氯代二苯并二英(2,3,7,8-tetrachlorodibenzo-p-dioxin,TCDD)诱导表达绿色荧光蛋白的细胞系XRE5-SGC-7901.将XRE5-SGC-7901细胞株暴露于不同浓度TCDD(0、250、500pg·mL-1)中,发现TCDD暴露组XRE5-SGC-7901细胞中绿色荧光蛋白的表达量显著升高,且与TCDD暴露浓度呈正相关.新构建的细胞株具有二英类化合物诱导表达绿色荧光蛋白的功能. |
| 关 键 词： | 二噁英类化合物 诱导表达载体 绿色荧光蛋白 |
| 收稿时间： | 2008-02-16 |
| 修稿时间： | 2008-03-31 |
The Establishment of a Gastric Cancer Cell Line Expressed Enhanced Green Fluorescent Protein (EGFP) Induced by Dioxin-like Chemicals |
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| WANG Chang,WANG Ying,LEI Yao,ZHANG Jie,ZHENG Ming-hui,LIANG Yong. The Establishment of a Gastric Cancer Cell Line Expressed Enhanced Green Fluorescent Protein (EGFP) Induced by Dioxin-like Chemicals[J]. Asian Journal of Ecotoxicology, 2008, 3(3): 244-249 | |
| Authors: | WANG Chang WANG Ying LEI Yao ZHANG Jie ZHENG Ming-hui LIANG Yong |
| Abstract: | Five joined XRE fragments and minimal CMV (Human cytomegalovirus) promoter were inserted into pEGFP-1 to generate a new vector pXRE5-EGFP. When human gastric cancer cell line SGC-7901 was stably transfected with this construct, the recombinant cell line XRE5-SGC-7901 expressed enhanced green fluorescent protein (EGFP) following stimulation with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). After exposed to different concentrations of TCDD (0, 250, 500pg·mL-1), the cell line expressed EGFP in dose-dependent manners. These results suggested that XRE5-SGC-7901 was a useful bioassay system for dioxin-like chemicals. |
| Keywords: | dioxin-like chemicals induced expression vector green fluorescent protein |
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