SPE-HPLC法检测环境水样中的痕量三氯卡班

建立了环境水样中三氯卡班(TCC)的预处理和测定方法。考察了3种固相萃取(SPE)小柱、5种洗脱液对TCC回收率的影响。结果表明,采用ENVI-18 SPE小柱、以乙酸乙酯/乙腈(1:1)为洗脱液、高效液相色谱仪-紫外检测器(HPLC-UV)检测,以蒸馏水为背景溶液TCC的加标回收率高达95%,仪器检出限与定量限分别为2.37与7.89μg/L。该方法用于实际环境水样,TCC加标浓度1~10μg/L,污水厂进水、出水与地表水中TCC的加标回收率分别在89.38%~96.90%、87.74%~94.34%与83.64%~94.61%之间,表明所建立的SPE-HPLC法适合城市生活污水和地表水中痕量TCC的检测。运用该方法测定实际环境水样中的TCC含量,集美污水处理厂进水与出水中TCC浓度分别为1.35与0.22μg/L;华大污水厂进水与出水中TCC浓度分别为1.05与0.53μg/L;白鹭湖水样中的TCC浓度为1.11μg/L。

Determination of trace triclocarban in environmental water samples by SPE-HPLC

A method for the pretreatment and determination of triclocarban(TCC) in environmental water samples was developed.Two factors that affect TCC recovery(3 kinds of solid phase extraction(SPE) columns and 5 kinds of eluents) were investigated.With the ENVI-18 as the SPE column and ethyl acetate/acetonitrile(1:1) as the eluent,the recovery rate of TCC was 95% by high performance liquid chromatography with UV-detection(HPLC-UV).The detection and quantitative limits of the instrument were 2.37 and 7.89μg/L,respectively with the distilled water as the background solution.The actual environmental water samples were also analyzed with the established method.Samples,which were collected from the influent and effluent of sewage plant and surface water,were spiked with 1~10μg/L TCC.The recovery rates were 89.38%~96.90%,87.74%~94.34% and 83.64%~94.61%,respectively.The results demostrated that the SPE-HPLC method was feasible for the detection of trace TCC in municipal wastewater and surface water.The method was used to determine TCC content in the actual environmental water samples.The TCC concentrations of influent and effluent of Jimei sewage treatment plant were 1.35 and 0.22μg/L,respectively.And that from the influent and effluent of Huaqiao University wastewater treatment plant were 1.05 and 0.53μg/L.The TCC concentration of water sample from the Egrets Lake of Huaqiao University was 1.11μg/L.