| Ames测试不确定性分析 |
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| 引用本文： | 刘波,金建玲,张辉,高培基.Ames测试不确定性分析[J].应用与环境生物学报,2007,13(5):726-730. |
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| 作者姓名： | 刘波 金建玲 张辉 高培基 |
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| 作者单位： | 山东大学微生物技术国家重点实验室,济南,250100 |
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| 摘 要： | Ames测试具有实验周期短、简便的优点,是目前普遍采用的检测化合物致突变性的初筛方法,但Ames测试仍有一定的不确定性.本文主要依据本实验室的研究结果分析了Ames测试中测试菌株、统计回复子数目的时间及2倍判定标准给测试结果带来的不确定性,对影响Ames测试结论存在假阳性及假阴性的因素做了进一步分析与讨论.图6表1参19
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| 关 键 词： | Ames测试 致突变物 致癌物 假阴性 假阳性 |
| 收稿时间： | 2006-08-28 |
| 修稿时间： | 2006-11-10 |
Uncertainty of Ames Test |
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| LIU Bo,JIN Jianling,ZHANG Hui,GAO Peiji.Uncertainty of Ames Test[J].Chinese Journal of Applied and Environmental Biology,2007,13(5):726-730. |
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| Authors: | LIU Bo JIN Jianling ZHANG Hui GAO Peiji |
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| Institution: | State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China |
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| Abstract: | Ames test was widely used to detect and predict the mutagenicity of chemicals,but it had many uncertain factors.In this article,some factors leading to uncertainty of the Ames test were revealed,such as the test strains,time to count the number of revertant colonies,two-fold rule calculating the possibility of mutagenicity and quantity of histidine in the tested materials.The potential factors resulting in false negative and false positive were investigated.Fig 6,Tab 1,Ref 19 |
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| Keywords: | Ames test mutagen carcinogen false negative false positive |
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