氧化铜纳米颗粒对水稻幼苗根系代谢毒性的研究

为阐明氧化铜纳米颗粒(CuO NPs)暴露对植物生长的影响,分别用CuO NPs悬浮液、氧化铜大颗粒(CuO BPs)悬浮液和硫酸铜(CuSO4·5H2O)溶液进行处理,以蒸馏水(d H2O)作为对照,水培水稻幼苗,研究了CuO NPs对水稻幼苗根系相关生理生化行为的影响.结果表明,不同处理对水稻幼苗根系的影响有很大差异,经CuO NPs悬浮液处理后,水稻幼根受损较严重,根系活力明显降低,根内丙二醛(MDA)含量和过氧化氢(H2O2)含量显著升高,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性明显增强.不同处理对水稻幼苗根的影响依次为:CuO NPs>CuSO4·5H2O>CuO BPs>d H2O.说明氧化铜纳米颗粒对水稻根的毒害作用不仅是由于释放离子引起的,纳米颗粒本身也起着重要作用,氧化损伤可能是CuO NPs的主要致毒机制之一.

Phytotoxicity of Copper Oxide Nanoparticles to Metabolic Activity in the Roots of Rice

To study the toxicity of copper oxide nanoparticles (CuO NPs) on plant growth, the physiological and biochemical activities of rice (Oryza sativa L.) seedlings were detected under different kinds of treatments (CuO NPs suspension, CuO BPs suspension and CuSO₄·5H₂ O solution), using distilled water (d H₂O) culture as contrast. The results showed that the response of rice roots to four treatments was very different. After treated with CuO NPs suspension, the rice roots were seriously damaged, the root activity obviously decreased, the MDA and H₂O₂ content rose, and the activity of SOD and POD apparently increased. The effect of different treatments on rice roots followed the order of CuO NPs>CuSO₄·5H₂ O>CuO BPs>d H₂O. The results demonstrated that CuO NPs had a serious effect on the root growth of rice, the toxicity of nanoparticles on rice root was not only caused by the release of Cu ion, the particles also played an important role, and that oxidative damage might be one of the main toxic mechanisms of copper oxide nanoparticles.