| 固定化原油降解菌的制备及其性能研究 |
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| 引用本文： | 张秀霞,秦丽姣,吴伟林,赵朝成. 固定化原油降解菌的制备及其性能研究[J]. 环境工程学报, 2010, 4(3): 659-664 |
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| 作者姓名： | 张秀霞 秦丽姣 吴伟林 赵朝成 |
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| 作者单位： | 中国石油大学(华东)环境科学与工程系,青岛,266555 |
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| 基金项目： | 中国石油天然气集团公司中青年创新基金项目（06E1023）;中国石油科技创新基金（2009D-5006-07-01） |
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| 摘 要： | 从石油污染土壤中筛选出的石油降解菌Y-13对石油的降解率为57.45%,经初步鉴定为芽孢杆菌属,以Y-13为固定化对象,选择聚乙烯醇(PVA)、海藻酸钠(SA)为主要载体材料,以胜利原油为反应底物,研究了固定化微球的制备和运行条件对微球性能的影响,实验确定了PVA-H3BO3包埋法制备固定化微球的最佳条件,最高降解率为79.04%;对PVA-H3BO3微球的性能测定结果表明:这种固定化微球具有良好的机械强度、弹性和渗透性、较高的比表面积和孔容;固定化微球比游离细菌对外界的适应性要强,适宜pH值、接种量和活化时间分别为7.5、1∶10和21 h。
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| 关 键 词： | 固定化微生物 制备 石油降解率 性能 |
Preparation of immobilized crude oil degrading strain and research of its property |
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| Zhang Xiuxi,Qin Lijiao,Wu Weilin and Zhao Chaocheng. Preparation of immobilized crude oil degrading strain and research of its property[J]. Techniques and Equipment for Environmental Pollution Control, 2010, 4(3): 659-664 |
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| Authors: | Zhang Xiuxi Qin Lijiao Wu Weilin Zhao Chaocheng |
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| Affiliation: | Department of Environmental Science and Engineering, China University of Petroleum, Qingdao 266555, China,Department of Environmental Science and Engineering, China University of Petroleum, Qingdao 266555, China,Department of Environmental Science and Engineering, China University of Petroleum, Qingdao 266555, China and Department of Environmental Science and Engineering, China University of Petroleum, Qingdao 266555, China |
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| Abstract: | A petroleum hydrocarbon degrading strain Y-13 was selected from the soil which contaminated by oil,its degradation rate was 57.45%,and it was the Bacillus by the preliminary determination.Taking Y-13 for immobilized and choosing polyvinyl alcohol(PVA),sodium alginate(SA) as the main carrier material,crude oil from Shengli oil field was selected to be response substance.Effect of preparation conditions and running conditions on characteristics of immobilized balls were studied.The optimum imbedding condition... |
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| Keywords: | immobilization microbe preparation technology oil degradation rate characteristics of immobilized balls |
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