Uptake and Metabolism of TNT and GTN by Plants Expressing Bacterial Pentaerythritol Tetranitrate Reductase |
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| Authors: | Nerissa K Hannink Susan J Rosser Christopher E French Neil C Bruce |
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| Institution: | (1) Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, U.K;(2) Present address: Institute of Cell and Molecular Biology, University of Edinburgh, Darwin Building, King's Buildings Mayfield Road, Edinburgh, EH9 3JR, U.K;(3) Present address: Centre for Novel Agricultural Products, Department of Biology, University of York, P.O. Box 373, York, YO10 5YW, U.K. (author for correspondence |
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| Abstract: | The manufacture and improper disposal of explosives has resulted in a significant amount of land requiring remediation. The compound 2,4,6-trinitrotoluene (TNT) is the most persistent and toxic of the explosive pollutants with current treatment methods being energy intensive and costly. Bacterial enzymes such as pentaerythritol tetranitrate reductase (PETNR) from Enterobacter cloacae PB2 have been found to have activity against TNT; however, microbes often lack the biomassnecessary for remediation applications. The PETNRgene (onr1) was transformed into tobacco plants in an attempt to combine the metabolic diversity of microbes with the sequestering properties of plants. The resulting transgenic plants were shown to have enhanced tolerance to TNT during germination and as seedlings. Phytoremediation applications with these plants may provide an alternative treatment of TNT contamination. |
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| Keywords: | aromatics explosives glycerol trinitrate nitroglycerin (GTN) nitrate esters plants phytoremediation 2 4 6-trinitrotoluene (TNT) |
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