稀有鮈鲫Dmrt基因家族13个成员的克隆与序列分析

已经发现果蝇Doublesex、线虫Mab-3、青DMRT1Y/DMY和人类DMRT1等性别决定与分化基因均含有一个具有DNA结合能力的保守基序--DM结构域,对性别决定和性别分化具有调控功能.利用简并PCR,从稀有鲫基因组DNA中克隆了13个具有不同DM结构域的Dmrt基因家族成员.基于DM保守基序,建立了各物种的进化树.结果表明,稀有鲫基因组存在多个Dmrt基因成员,该基因在脊椎动物和非脊椎动物中具有高度保守性,是一种理想的环境内分泌干扰物研究的分子模型,在分子生态毒理学研究中具有很好的应用前景.

Clone and Sequence Analysis of Thirteen Members of Dmrt Genes in Rare Minnow

Sex determining genes Mab-3 of C.elegans and Doublesex of Drosophila, human Doublesex related gene DMRT1 and medaka sex-determining gene DMRT1Y/DMY contain a common DNA binding motif called DM(Doublesex and Mab-3)domain, both of which regulate similar aspects of sexual development. We amplified genomic DNA of the rare minnow using the DM degenerate primers and detected an approximately 140bp band. After cloned into p MD-19 vector and sequenced, thirteen sequences showed high homology with the DM domain. Based on similarities of amino acid sequences of the DM domain, Dmrt protein sequences from many species were included in a phylogenetic tree. The results further revealed the unexpected complexity and the evolutionary conservation of the DM domain gene family in both invertebrates and vertebrates. Dmrt genes could be used as a molecular model to study the ecotoxicology of endocrine disruptors in the future.