超高效液相色谱串联质谱法测定水体中17种抗生素

建立了一种超高效液相色谱串联质谱法同时测定饮用水源中17种超痕量水平抗生素的分析方法。采用Oasis HLB柱对500 mL pH=5含目标物的水样进行富集，干燥15 min后，用5%氨水甲醇洗脱，氮吹后定容至1 mL。用C₁₈柱吸附目标物，并用0.1%甲酸水和甲醇对目标物进行分离，采用正离子电喷雾电离模式在多反应监测通道中对目标物同时进行分析。该方法检出限为0.07~0.78 ng/L，超痕量水平下回收率范围为75.8%~108%。该方法灵敏度高，精密度为1.8%~15.0%，适用于同时测定饮用水源等水体中的抗生素。

Determination of 17 Antibiotics in Drinking Water Sources in Zhejiang Province by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

An analytical method was developed for the simultaneous determination of 17 ultra-trace levels of antibiotics in drinking water sources by ultra-performance liquid chromatography-tandem mass spectrometry.Oasis HLB column was used to enrich 500 mL water samples containing the target compounds with pH=5.After drying for 15 min,elution was conducted with 5% ammonia methanol,and then blow the extracted liquid nitrogen to 1 mL.The target was adsorbed by C₁₈ column and separated by 0.1% formic acid water and methanol.The target was analyzed simultaneously in the multi-reaction monitoring channel by using positive ion electrospray ionization mode.The detection limit was 0.07-0.78 ng/L,and the recovery at ultra-trace level was between 75.8%-108%.The method has high sensitivity,high efficiency and stable precision of 1.8%-15.0%,which can be used for simultaneous determination of antibiotics in drinking water sources,surface water and other water bodies.