| 显微分光光度计对水体有机污染物诱导的转化细胞DNA含量的分析 |
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| 引用本文： | 张俊然,朱惠刚.显微分光光度计对水体有机污染物诱导的转化细胞DNA含量的分析[J].中国环境科学,1997,17(3):0-0. |
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| 作者姓名： | 张俊然 朱惠刚 |
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| 作者单位： | 上海医科大学环境卫生学教研室 上海200032 |
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| 摘 要： | 在水源水及自来水有机提取物诱导人胚肺（BKM）细胞恶性转化基础上，运用MPV－SP型显微分光光度计对Ⅲ型转化灶中细胞的DNA含量进行了分析。结果表明：水样在实验剂量范围内可不同程度地诱导细胞转化灶的形成，并有明显的剂量反应关系。与源水相比，自来水有较强的潜在致癌性，从Ⅲ型转化灶克隆中挑取的细胞DNA含量增高，与阴性对照比有显著性差异，说明转化细胞形态学改变与细胞DNA含量的改变有一定的关系。
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| 关 键 词： | 细胞转化实验 人胚肺细胞 自来水 水源水 显微分光光度计 |
| 收稿时间： | 1900-01-01; |
Analysis on DNA content of trasformation cells induced by extracts of water using microspectrophotometer |
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| Zhang Junran and Zhu Huigang.Analysis on DNA content of trasformation cells induced by extracts of water using microspectrophotometer[J].China Environmental Science,1997,17(3):0-0. |
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| Authors: | Zhang Junran and Zhu Huigang |
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| Institution: | Zhang Junran and Zhu Huigang Department of Environmental Health,Shanghai Medical University,Shanghai 200032 |
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| Abstract: | Based on the study of the potential carcinogenicity of source water and drinking water of a certain city by BKM cells maligmant transformation, the DNA content of focic derived cells were analysised using microspectrophotometer.The results showed that extracts of both tap water and drinking water could induce BKM cell malignant transformation. Comparing with negative cells, a higher absorbance value representing DNA content of transformative cells was observed.There is a certain relationship between the cell morphological change and the DNA content. |
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| Keywords: | maligmant transformation BKM cell drinking water source water microspectrophotometer |
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