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Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish |
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| Authors: | Koji Yachiguchi Noriko Matsumoto Yuki Haga Motoharu Suzuki Chisato Matsumura Masahiro Tsurukawa Toshihiro Okuno Takeshi Nakano Kimi Kawabe Kei-ichiro Kitamura Akira Toriba Kazuichi Hayakawa Vishwajit S. Chowdhury Masato Endo Atsuhiko Chiba Toshio Sekiguchi Masaki Nakano Yoshiaki Tabuchi Takashi Kondo Shigehito Wada Hiroyuki Mishima Atsuhiko Hattori Nobuo Suzuki |
| Affiliation: | 1. Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Ogi, Noto-cho, Housu-gun, Ishikawa, 927-0553, Japan 2. Hyogo Prefectural Institute of Environmental Sciences, Kobe, Hyogo, 654-0037, Japan 3. Faculty of Pharmaceutical Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan 4. Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kodatsuno, Ishikawa, 920-0942, Japan 5. International Education Center, Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan 6. Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, 108-8477, Japan 7. Department of Materials and Life Sciences, Sophia University, Tokyo, 102-8554, Japan 8. Department of Biology, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Chiba, 272-0827, Japan 9. Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, Sugitani, Toyama, 930-0194, Japan 10. Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama, 930-0194, Japan 11. Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Toyama, Sugitani, Toyama, 930-0194, Japan 12. Department of Human Life Sciences, Kochi Gakuen College, Kochi, 780-0955, Japan |
| Abstract: | To analyze the effect of polychlorinated biphenyl (PCB) 118 on fish bone metabolism, we examined osteoclastic and osteoblastic activities, as well as plasma calcium levels, in the scales of PCB (118)-injected goldfish. In addition, effect of PCB (118) on osteoclasts and osteoblasts was investigated in vitro. Immature goldfish, in which the endogenous effects of sex steroids are negligible, were used. PCB (118) was solubilized in dimethyl sulfoxide at a concentration of 10 ppm. At 1 and 2 days after PCB (118) injection (100 ng/g body weight), both osteoclastic and osteoblastic activities, and plasma calcium levels were measured. In an in vitro study, then, both osteoclastic and osteoblastic activities as well as each marker mRNA expression were examined. At 2 days, scale osteoclastic activity in PCB (118)-injected goldfish increased significantly, while osteoblastic activity did not change significantly. Corresponding to osteoclastic activity, plasma calcium levels increased significantly at 2 days after PCB (118) administration. Osteoclastic activation also occurred in the marker enzyme activities and mRNA expressions in vitro. Thus, we conclude that PCB (118) disrupts bone metabolism in goldfish both in vivo and in vitro experiments. |
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