| 节织纹螺饲喂富集河豚毒素及相关差异蛋白表达的研究 |
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| 引用本文： | 苏捷,许旻,姜琳琳,吴靖娜,张农,刘智禹.节织纹螺饲喂富集河豚毒素及相关差异蛋白表达的研究[J].生态毒理学报,2014,9(6):1060-1067. |
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| 作者姓名： | 苏捷 许旻 姜琳琳 吴靖娜 张农 刘智禹 |
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| 作者单位： | 1. 福建省水产研究所,厦门,361012;中山大学,生命科学学院,广州,510275
2. 福建省水产研究所,厦门,361012 |
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| 基金项目： | Supported by Overseas funds for Science and Technology and Scientific, Marine High-tech Industry Project in Fujian Province（[2013]027）, and Technical Innovation Fund of Xiamen City, China(3502Z20122006) |
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| 摘 要： | 我国东南沿海经常有食用含有河豚毒素节织纹螺的中毒事件发生,含有河豚毒素的动物对河豚毒素有很高的耐受能力,并对环境中的河豚毒素具有趋食性.织纹螺可以富集食物中的河豚毒素,但织纹螺体内的与河豚毒素富集相关的蛋白质还不清楚.为此我们收集了无毒的织纹螺,通过河豚毒素富集实验和蛋白质组学分析,发现在有毒织纹螺中有4种蛋白质表达量显著增多,飞行质谱(MALDI-TOF-MS)鉴定分析它们分别是actin、actin2、beta-actin、膜泡ATP合酶B亚基.分析表明这些蛋白可能与河豚毒素在织纹螺体内富集、传递有关.
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| 关 键 词： | 织纹螺 河豚毒素 富集 双向电泳 传递 |
| 收稿时间： | 2014/2/27 0:00:00 |
| 修稿时间： | 2014/6/12 0:00:00 |
Tetrodotoxin Enrichment by Feeding Toxin to the Snail Nassarius semiplicata and Proteomic Analysis of Differentially Expressed Proteins |
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| Su Jie,Xu Min,Jiang Linlin,Wu Jingn,Zhang Nong and Liu Zhiyu.Tetrodotoxin Enrichment by Feeding Toxin to the Snail Nassarius semiplicata and Proteomic Analysis of Differentially Expressed Proteins[J].Asian Journal of Ecotoxicology,2014,9(6):1060-1067. |
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| Authors: | Su Jie Xu Min Jiang Linlin Wu Jingn Zhang Nong and Liu Zhiyu |
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| Institution: | 1. Fisheries Research Institute of Fujian, Xiamen 361012, China
2. Sun Yat-sen University, School of Life Sciecne, Guangzhou 510275, China;Fisheries Research Institute of Fujian, Xiamen 361012, China;Fisheries Research Institute of Fujian, Xiamen 361012, China;Fisheries Research Institute of Fujian, Xiamen 361012, China;Fisheries Research Institute of Fujian, Xiamen 361012, China;Fisheries Research Institute of Fujian, Xiamen 361012, China |
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| Abstract: | |
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| Keywords: | Nassarius tetrodotoxin enrichment two-dimensional electrophoresis transportation |
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