六种表面活性剂对斑马鱼胚胎发育的毒性效应

为评价六种常见农药表面活性剂对水生生物的风险,采用斑马鱼胚胎发育技术,研究其对斑马鱼胚胎的致死效应和致畸效应.结果表明,NP-10、OP-10、农乳700、农乳602、农乳1602、宁乳33对斑马鱼胚胎的致死中浓度分别为16.44、21.13、55.86、8446、109.90、120.08 mg·L-1,其中NP-10对斑马鱼胚胎急性毒性最大,宁乳33毒性最低;随着染毒剂量的增加,斑马鱼胚胎孵化率逐渐降低,致畸率逐渐提高,六种表面活性剂显示出相似的趋势,处理浓度与胚胎孵化率(致畸率)之间存在剂量-效应关系;NP-10、OP-10、农乳700处理组均诱导斑马鱼胚胎出现躯干侧翻、游囊关闭、躯干弯曲症状;农乳602、农乳1602、宁乳33处理组出现躯干侧翻、游囊关闭症状.

The Toxicity Effects of Six Pesticide Surfactants on Embryo Development of Zebrafish

For the risk assessment of six common pesticides surfactants on aquatic organisms, the lethal effects and teratogenic effect of six pesticides surfactants on zebrafish embryo was studied using a zebrafish embryo development technique. Results showed that the LC₅₀ of NP-10, OP-10, 700#, 602# , 1602#, 33 # was16.44, 21.13, 55.86, 84.46, 109.90, 120.08 mg ? L^-1 respectively. NP-10 showed the maximum acute toxicity to zebrafish embryos while 33# showed the minimum acute toxicity. The hatching rate decreased gradually and the malformation rate increased gradually with the dose increasing. The six surfactants showed a similar trend and there was a dose effect relationship between concentration and embryo hatching rate (malformation rate). Juvenile fish exhibited teratogenic characteristics of body-lean to one side, uninflated swim bladder, bent spine after being exposed to NP-10, OP-10 and 700# while exhibited teratogenic characteristics of body-lean to one side, uninflated swim bladder after being exposed to 602#, 1602# and 33#.