The influence of storage on the morphology and physiology of phthalic acid-degrading aerobic granules

The precultured aerobic granules with special degradabilities could be used as a feasible bioseed for enhancement of aerobic granulation systems. In practice, the storage stability, physicochemical characteristics, and recovering efficiency of granules are crucial for a long-distance transportation and successful application. In this study, phthalic acid (PA)-degrading aerobic granules were cultivated and stored for 8 wk at 4 °C. The granular size, settling ability as well as structure integrity was found stable during the storage period. It was observed that the upper 1/3 part of granules stored in the reagent bottle turned to black color, while the lower 2/3 part granules did not significantly change color (brown–yellow) after the 8-wk storage. The black and brown–yellow color PA-degrading granules were manually separated and re-inoculated into two identical sequencing batch reactors for reviving the PA degradation capability. After a 7 d operation, both black and yellow granules restored their activities to the levels before storage, in terms of total organic carbon removal efficiency (100%), specific oxygen uptake rate (59 mg g VSS^?1 h^?1), and adenosine triphosphate content (0.016 mg g VSS^?1). This study demonstrated that aerobic granules grown on a complex substrate could tolerate storage conditions and rapidly restored their bioactivities toward the target pollutant. The results also shed the light on the future application of precultured aerobic granules with unique functions for biodegradation and bioremediation purpose.