| 石油降解菌Pseudomonas stutzeri TH-31的分离与降解条件优化 |
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| 引用本文： | 郝婧, 高磊, 吴春旭, 张鸿涛, 王玉双, 陈兆林, 赵鑫. 石油降解菌Pseudomonas stutzeri TH-31的分离与降解条件优化[J]. 环境工程学报, 2015, 9(4): 1771-1777. doi: 10.12030/j.cjee.20150441 |
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| 作者姓名： | 郝婧 高磊 吴春旭 张鸿涛 王玉双 陈兆林 赵鑫 |
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| 作者单位： | 1. 清华大学环境学院, 北京 100084; 4. 北京建科宏达节能技术推广中心, 北京 100835; 2. 北京国环清华环境工程设计研究院有限公司, 北京 100084; 3. 东北大学资源与土木工程学院, 沈阳 110004 |
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| 基金项目： | “十一五”国家科技支撑计划重点项目(2008BAC43B03) |
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| 摘 要： | 使用稀释富集法,从大港油田采油废水处理站生化池中定向快速的分离出多株高效石油降解菌株。对分离获得的1株优势菌株进行生理生化和分子生物学鉴定,显示属于Pseudomonas stutzeri的1个新菌株,命名为Pseudomonas stutzeri TH-31。通过批次实验,对菌株TH-31的生长条件和石油降解条件进行了优化,经过条件优化,P.stutzeri TH-31在初始pH 7,原油投加量为300 mg/L,35℃培养5 d后,获得最高石油烃降解率92.7%。
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| 关 键 词： | 石油降解菌 降解率 Pseudomonas stutzeri 稀释富集 分离 条件优化 |
| 收稿时间： | 2014-05-13 |
Isolation and optimization of a crude-oil-degrading bacteria Pseudomonas stutzeri TH-31 |
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| Hao Jing, Gao Lei, Wu Chunxu, Zhang Hongtao, Wang Yushuang, Chen Zhaolin, Zhao Xin. Isolation and optimization of a crude-oil-degrading bacteria Pseudomonas stutzeri TH-31[J]. Chinese Journal of Environmental Engineering, 2015, 9(4): 1771-1777. doi: 10.12030/j.cjee.20150441 |
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| Authors: | Hao Jing Gao Lei Wu Chunxu Zhang Hongtao Wang Yushuang Chen Zhaolin Zhao Xin |
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| Affiliation: | 1. School of Environment, Tsinghua University, Beijing 100084, China; 4. Beijing Jianke-Hongda Sustainable Technology Promotion Co.Ltd., Beijing 100835, China; 2. Beijing Guohuan Tsinghua Environmental Engineering Design & Research Institute, Beijing 100084, China; 3. College of Resource & Civil Engineering, Northeastern University, Shenyang 110004, China |
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| Abstract: | Dilution enrichment method was used to directly and fast isolate high efficient oil degrading bacteria from the biochemical pool of Dagang Oilfield Wastewater Treatment Plant.One of the dominant strains was identified to be a new strain of Pseudomonas stutzeri through physiological,biochemical and molecular biological identification,and named Pseudomonas stutzeri TH-31.Through batch culture experiments,the growth and oil degradation conditions of strain TH-31 were optimized.A maximum petroleum hydrocarbon degradation rate of 92.7% by P.stutzeri TH-31 was obtained with the initial pH 7,crude oil dosage of 300 mg/L,and cultured at 35℃ for 5 d. |
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| Keywords: | oil degrading bacteria degradation rate Pseudomonas stutzeri dilution enrichment isolation condition optimization |
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