Detection, isolation, and identification of cadmium-resistant bacteriabased on PCR-DGGE |
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Authors: | HU Qing DOU Min-n QI Hong-yan XIE Xiang-ming ZHUANG Guo-qiang YANG Min |
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Affiliation: | 1. Department of Environmental Biotechnology,Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences,Beijing 100085,China 2. Department of Environmental Biotechnology,Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences,Beijing 100085,China;Biology Science and Technology College,Beijing Forestry University,Beijing 100083,China 3. Biology Science and Technology College,Beijing Forestry University,Beijing 100083,China |
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Abstract: | This study focused on the screening of cadmium-resistant bacterial strains from Pb-Zn tailing.We investigated the diversity of microbial community inhabiting Dong-san-cha Pb-Zn tailing in Beijing,China,by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene of bacterial strain,and found two dominant strains in the DGGE profile.Using special culture media,We isolated two strong cadmium-resistant bacterial strains.On the basis of morphological,physiological,and biochemical characteristics,BIOLOG,and 16S rDNA sequencing,the two strains were identified as Bacillus cereus and Enterobaeter cloacae.Minimal inhibitory concentrations (MICs) of heavy metals for the bacteria were determined.E.cloacae showed higher MIC values for heavy metals and a larger range of antibiotic resistance than B.cereus. |
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Keywords: | Bacillus cereus Enterobacter cloacae PCR-DGGE cadmium resistance antibiotic resistance Detection isolation based bacteria Bacillus cereus values range antibiotic resistance heavy metals MICs Minimal inhibitory concentrations cloacae rDNA sequencing BIOLOG biochemical characteristics basis isolated |
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