Construction and application of the Synechocystis sp. PCC6803-ftnA in microbial contamination control in a coupled cultivation and wastewater treatment |
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Authors: | Yalei Zhang Chunmin Zhang Xuefei Zhou Zheng Shen Fangchao Zhao Jianfu Zhao |
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Affiliation: | 1 State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze Water Environment of Ministry of Education, Tongji University, Shanghai 200092, China;2 Water Conservancy Development Research Center, Taihu Basin Authority of Ministry of Water Resources, Shanghai 200434, China |
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Abstract: | Inspired by iron fertilization experiments in HNLC (high-nitrate, low-chlorophyll) sea areas, we proposed the use of iron-rich engineered microalgae for microbial contaminant control in iron-free culture media. Based on the genome sequence and natural transformation system of Synechocystis sp. PCC6803, ftnA (encoding ferritin) was selected as our target gene and was cloned into wild-type Synechocystis sp. PCC6803. Tests at the molecular level confirmed the successful construction of the engineered Synechocystis sp. PCC6803-ftnA. After Fe3 +-EDTA pulsing, the intracellular iron content of Synechocystis sp. PCC6803-ftnA was significantly enhanced, and the algae was used in the microbial contamination control system. In the coupled Synechocystis sp. PCC6803-ftnA production and municipal wastewater (MW, including Scenedesmus obliquus and Bacillus) treatment, Synechocystis sp. PCC6803-ftnA accounted for all of the microbial activity and significantly increased from 70% of the microbial community to 95%. These results revealed that while the stored iron in the Synechocystis sp. PCC6803-ftnA cells was used for growth and reproduction of this microalga in the MW, the growth of other microbes was inhibited because of the iron limitation, and these results provide a new method for microbial contamination control during a coupling process. |
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Keywords: | Synechocystis PCC6803-ftnA Municipal wastewater treatment Microbial contaminants |
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