成团泛菌(Pantoea agglomerans)对镉胁迫下蒌蒿耐受性的影响

为探讨功能微生物对镉(Cd)胁迫下蒌蒿耐受性的影响,以成团泛菌(Pantoea agglomerans)J2为对象,通过盆栽试验,研究不同Cd处理水平下J2菌对蒌蒿生长、生理、Cd富集转运与根际微生态的影响。结果表明:J2菌可显著促进蒌蒿株高、地上部干重、地下部干重、总生物量及根冠比的增加。在J2菌的作用下,蒌蒿叶绿素a、叶绿素b和总叶绿素含量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性均显著增加,丙二醛(MDA)含量明显减少。J2菌可显著促进蒌蒿对Cd的富集转运,蒌蒿地上部、地下部与总Cd含量均显著增加,地上部生物富集系数(BCF)明显大于地下部,转运系数(TF)相比于对照增加了7.5%~37.4%。J2菌可显著增加蒌蒿根际土壤细菌、放线菌数量及微生物总量,明显减少真菌数量。研究表明成团泛菌J2对蒌蒿生长、生理、Cd富集转运与根际微生态可产生积极影响,在一定程度上可提高蒌蒿植株对Cd的耐受性。

Effects of Pantoea agglomerans on Tolerance of Artemisia selengensis under Cadmium Stress

To explore the effects of functional microorganism on Artemisia selengensis tolerance under cadmium stress, this study examined the effects of strain J2 on growth, physiology, cadmium concentration and translocation, rhizosphere micro-ecology of Artemisia selengensis under different concentrations of cadmium by pot experiment. The results indicated that Pantoea agglomerans J2 distinctly enhanced the plant height, shoot dry weight, root dry weight, the total dry weight and root-shoot ratio of Artemisia selengensis under cadmium stress. Strain J2 distinctly increased the contents of chlorophyll a, chlorophyll b and the total chlorophyll as well as the activities of superoxide dismutase (SOD), peroxidase (POD)and catalase (CAT), whereas significantly reduced the malondialdehyde (MDA) contents of Artemisia selengensis under cadmium stress. Pantoea agglomerans J2 distinctly promoted Artemisia selengensis to concentrate and translocate cadmium, and shoot cadmium contents, root cadmium contents and the total cadmium contents were significantly increased by Pantoea agglomerans J2 treatment. Bioconcentration factors (BCF) of cadmium in shoot were significant greater than in root. Moreover, translocation factors (TF) of Artemisia selengensis increased by 7.5%-37.4% under cadmium stress compared with the control. Pantoea agglomerans J2 significantly improved the number of bacteria and actinomyces and the total number of microorganisms along with distinctly reducing the number of fungi of Artemisia selengensis rhizosphere soil under cadmium stress. The study suggested that Pantoea agglomerans J2 was conducive to improve not only growth, physiology, cadmium concentration and translocation as well as rhizosphere micro-ecology of Artemisia selengensis under cadmium stress, but also to some extent, Artemisia selengensis tolerance to cadmium.