多氯联苯(PCBs)胁迫下鲫鱼肝脏EROD酶活性与血清性激素含量的相关性研究

以鲫鱼(Carassius auratus)为试验鱼类,研究其暴露于不同浓度的PCBs(多氯联苯)后鱼肝组织中EROD酶活性和血清性激素含量的动态变化,探讨了2者间的相关性。结果表明,鲫鱼在PCBs中暴露后,其肝脏组织中EROD酶被诱导,酶活性随PCBs浓度增大而增强,呈明显的剂量-效应关系;EROD酶活性随暴露时间延长而上升,10 d后达平衡;鲫鱼血清中睾酮含量随PCBs浓度增大和暴露时间延长呈下降趋势,但雌二醇含量则显著上升,表明PCBs对鱼类具有环境雌激素效应;在一定浓度范围内,EROD酶活性与血清睾酮含量呈负相关,与血清雌二醇含量呈正相关。因此可用鱼肝EROD酶和血清性激素含量的协同变化作为污染生物标志物来评价PCBs的早期污染生态效应。

Correlation Between EROD Activity in Liver and Sex Hormone in Blood Serum of Carassius auratus Under Stress of Polychlorinated Biphenyls

Dynamic change was studied in EROD activity in the liver and sex hormone in the blood serum of Carassius auratus.After exposure to different concentrations of polychlorinated biphenyls(PCBs),and the correlation between them was also discussed.Results show that EROD in the liver of Carassius auratus was induced and increased in activity with the increasing of PCBs concentration,demonstrating that the former is positively related to the latter.The activity of EROD also rose with the time,reaching dynamic balance after 10 days.Compared with CK,the content of testosterone in the blood serum of the fish rose with the concentration of PCBs,but declined with the exposure time,while the content of estradiol therein rose significantly with both the concentration and the time,indicating that PCBs have certain environmental estrogen effect on(fishes).The results also indicate that within a certain range of concentrations,EROD activity was negatively related to testosterone content,but positively related to estradiol content in blood serum of the fish.Concurrent changes in EROD in the liver and sex hormone in the blood serum of fish could be cited as the bio-indicator to evaluate ecological effect of initial PCBs pollution.