接种AM真菌对压实土壤柠条叶片生理与光合特性的影响

以矿区常用复垦植物——柠条作为供试物种，采用室内实验模拟矿区压实土壤，研究接种AM真菌与土壤压实对柠条叶片生理生化和光合特性的影响。结果表明:土壤压实导致柠条叶片SOD、POD、CAT活性下降、脯氨酸、可溶性糖与可溶性蛋白含量降低，叶片相对电导率、丙二醛含量显著升高。接种AM真菌有助改善压实土壤中柠条叶片生理特性，增强光合作用。与单纯压实处理相比，接种AM真菌使压实处理柠条抗氧化酶SOD、POD、CAT活性分别提高1.34，1.65，1.51倍；可溶性糖、可溶性蛋白含量分别升高33.90%，5.30%，脯氨酸含量降低53.20%；叶片相对电导率、丙二醛含量显著降低15.58%、19.98%；激素调节物质IAA、CTK、GA激素分泌分别增加18.39%、45.19%、14.40%，ABA降低34.33%；叶片净光合速率、气孔导度和蒸腾速率有明显升高。表明接种AM真菌能够提高柠条抗氧化酶活性、调节渗透物质和激素分泌，降低细胞膜脂过氧化水平，维持细胞结构稳定，促进柠条光合作用增强，提高柠条的生产力。在土壤压实严重的矿区，可以通过接种AM真菌提高柠条抗压实胁迫的能力，以提高压实土壤矿区植被恢复质量。

EFFECTS OF AM FUNGUS INOCULATION ON PHYSIOLOGICAL AND PHOTOSYNTHETIC CHARACTERISTICS OF CARAGANA LEAVES UNDER SOIL COMPACTION STRESS

The effect of AM fungus inoculation on physiological, biochemical and photosynthetic characteristics of caragana leaves under soil compaction stress was studied. The results showed that the soil compaction led to the antioxidant enzymes activity of SOD, POD, CAT decreased, the content of proline, soluble sugar and soluble protein decreased, and the relative electrical conductivity and malondialdehyde increased significantly. Inoculation of AM fungus could improve the physiological characteristics of caragana leaves and enhance photosynthesis. Under soil compaction stress, the activity of antioxidant enzyme SOD, POD and CAT in caragana increased by 1.34, 1.65, 1.51 after AMF inoculation. The content of soluble sugar and soluble protein increased by 33.90% and 5.30% respectively, and the content of proline decreased by 53.20%. The relative conductivity and malondialdehyde content of the leaves decreased significantly by 15.58% and 19.98%. The secretion of hormone regulating substances IAA, CTK and GA increased by 18.39%, 45.19% and 14.40%, and ABA decreased by 34.33% with the inoculation of AMF. The net photosynthetic rate, stomatal conductance and transpiration rate of leaves were significantly increased. The results showed that the inoculation of AMF could improve antioxidant enzyme activity, regulate osmotic substance and hormone secretion, reduce lipid peroxidation level of cell membrane, maintain cell structure stability, promote photosynthesis and increase productivity. In the mining area with serious soil compaction, the inoculation of AMF could improve the ability of caragana to resist compaction stress, and improve the quality of vegetation restoration in mining areas.