基于EEM与高通量技术分析中药渣投加对餐厨垃圾堆肥的影响

以中药渣作为调理剂与外加碳源,利用三维荧光(EEM)考察了不同质量配比对餐厨垃圾与城市污泥共堆肥过程腐熟程度的影响,并采用高通量测序技术对不同堆体中的微生物群落进行探究.EEM分析结果表明:中药渣的添加可加快堆体腐熟进程,且随着投加量的增加,效果不断增强.而通过高通量测序可知:随着中药渣投加量的增加,堆体中微生物的生物...

ANALYSIS OF EFFECT OF ADDITION OF CHINESE HERBAL RESIDUE ON FOOD WASTE COMPOSTING BASED ON EEM AND HIGH-THROUGHPUT SEQUENCING

Using Chinese herbal residue as a conditioner and an external carbon source, the effect of different quality ratios on the composting process of food waste and municipal sludge were investigated by EEM spectra. In addition, the microbial communities in different piles were analyzed by using high throughput sequencing technology. The results of the EEM spectra showed that the addition of Chinese herbal residue accelerated the maturity process of composting. With the increase of the dosage, the effect was continuously enhanced. According to high throughput sequencing analysis, with the increase of the dosage of Chinese herbal residue, the microbial biodiversity in the piles gradually decreased, but the microbial community richness increased. The relative abundance of Proteobacteria decreased by adding Chinese herbal residue, while the relative abundance of Firmicutes increased. Meanwhile, adding a weight ratio 5% of Chinese herbal residue could improve the number of Clostridia, so as to promote the decomposition of cellulose in the piles, and avoid the local hardening of the piles caused by the excessive addition of Chinese herbal residue. In general, the addition of 5% Chinese herbal residue had best promoting effect on the composting process of food waste and municipal sludge.