氯代阻燃剂得克隆对孔石莼(Ulva pertusa)繁殖及早期发育的影响

得克隆(Dechlorane Plus,DP)是一种在全世界范围内广泛使用的氯代阻燃剂,具有潜在的毒性效应。但目前已有的生态毒理学数据还十分有限。本文选择分布广泛的大型绿藻孔石莼(Ulva pertusa)作为研究对象,探讨不同浓度DP对孔石莼繁殖细胞转化及早期发育的影响。结果表明,低浓度(10-6~10-8mol·L-1)DP暴露不同程度地影响孔石莼繁殖细胞的形成、释放及附着,在试验浓度范围内呈现一定的剂量效应关系。与空白对照组相比,DP处理(10-6mol·L-1)分别使繁殖细胞的形成率、释放率和附着率显著降低了76.76%、46.26%和85.64%。在丝状幼体阶段,DP处理组(10-8和10-7mol·L-1)幼体体长分别比对照组显著减少了32.74%和38.98%。结果表明,DP暴露抑制了孔石莼的繁殖及早期发育。基于绿藻繁殖特性及早期发育的生物学指标对DP暴露较为敏感,能够为海洋环境的DP生物学效应研究提供数据。

Effects of Dechlorane Plus on Reproduction and Early Development of Ulva pertusa

Dechlorane Plus (DP) is a highly chlorinated flame retardant which is globally ubiquitous. However, limited information is available on its toxicity in aquatic organisms, especially for marine algae. In this study, the common green macroalgae Ulva pertusa was used to evaluate possible eco-toxicological response, including the reproduction and early development with the low-dose DP exposure (10^-6 to 10^-8 mol L^-1). The results showed that DP inhibited the reproductive cells formation, release and adhesion in dose-effect manner. After exposure to DP in the concentration of 10^-6 mol L^-1, the rate of formation, release and adhesion were significantly decreased by 76.76%, 46.26% and 85.64%, respectively. The length of filaments was decreased by 32.74% and 38.98%, respectively with 10^-8 and 10^-7 mol L^-1of DP treatments. Our observations firstly indicated that DP disrupted the transformation of reproductive cells of U. pertusa as well as their early development. Reproductive cells formation, release and adhesion in macroalgae were sensitive biomarkers which can be used for assessing the marine ecological risks of DP.