|
|||||
|
|
A simple effective procedure for the determination of adenosine triphosphate in soils |
|
| Authors: | Q Y Bai L Zelles I Scheunert and F Korte |
| Institution: | a National Agroenvironmental Protection Institute, Fukang Road 31, Tianjin, People's Republic of China b Gesellschaft für Strahlen- und Umweltforschung mbH München, Institut für Ökologische Chemie, Ingolstädter Landstr. 1, D - 8042, Neuherberg, F.R.G. c Technische Universität München, Lehrstuhl für Ökologische Chemie, Am Löwentor, D - 8050, Freising-Weihenstephan, F.R.G. |
| Abstract: | A simple method for the extraction and determination of adenosine 5′-triphosphate (ATP) in soil is described. ATP was extracted by stirring with dimethyl sulphoxide (DMSO), followed by adding 0.01 M trisodium phosphate (Na3PO4) buffer solution for a further dispersion. The ATP content was quantitatively measured by luciferin-luciferase system employing a standard addition technique. The method was most efficient in comparison with 7 other extraction procedures. The recoveries of ATP in spiked soil were found to be approximately 100 %. ATP contents in 16 selected soils were in ranges of 0.76±0.05 – 7.79±0.83 μg/g (dry weight). A significant correlation between ATP amounts and biomasses in these soils was also observed. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |